Light-sheet Fluorescence Imaging to Localize Cardiac Lineage and Protein Distribution

Overview

Journal Sci Rep

Specialty Science

Date 2017 Feb 7

PMID 28165052

Citations 25

Authors

Yichen Ding

Juhyun Lee

Jianguo Ma

Kevin Sung

Tomohiro Yokota

Neha Singh

Mojdeh Dooraghi

Parinaz Abiri

Yibin Wang

Rajan P Kulkarni

Atsushi Nakano

Thao P Nguyen

Peng Fei

Tzung K Hsiai

Affiliations

Soon will be listed here.

Abstract

Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

Citing Articles

Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.

Zhu E, Li Y, Margolis S, Wang J, Wang K, Zhang Y View (Beijing). 2024; 5(5).

PMID: 39478956 PMC: 11521201. DOI: 10.1002/VIW.20230087.

A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.

Kramer S, Antarasen J, Reinholt C, Kisley L J Appl Phys. 2024; 136(9):091101.

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Light-Sheet Imaging to Reveal Cardiac Structure in Rodent Hearts.

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Management of autofluorescence in formaldehyde-fixed myocardium: choosing the right treatment.

Zhang Z, Fan H, Richardson W, Gao B, Ye T Eur J Histochem. 2023; 67(4).

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Tissue Clearing and Its Application in the Musculoskeletal System.

Zhan Y, Zhang S, Zhu S, Jiang N ACS Omega. 2023; 8(2):1739-1758.

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