Matching Target Dose to Target Organ

assays have become a mainstay of modern approaches to toxicology with the promise of replacing or reducing the number of tests required to establish benchmark doses, as well as increasing mechanistic understanding. However, matching target dose to target organ is an often overlooked aspect of assays, and the calibration of exposure against benchmark doses is often ignored, inadvertently or otherwise.  An example of this was recently published in by Wagner (2016), where neural stems cells were used to model the molecular toxicity of lead.  On closer examination of the work, the doses used in media reflected lead doses that would be at the highest end of lead toxicity, perhaps even lethal.  Here we discuss the doses used and suggest more realistic doses for future work with stem cells or other neuronal cell lines.