Enhancement of Furan Aldehydes Conversion in by Elevating Dehydrogenase Activity and Cofactor Regeneration

Overview

Journal Biotechnol Biofuels

Publisher Biomed Central

Specialty Biotechnology

Date 2017 Feb 7

PMID 28163781

Citations 12

Authors

Xia Wang

Qiuqiang Gao

Jie Bao

Affiliations

Soon will be listed here.

Abstract

Background: Furfural and 5-hydroxymethylfurfural (HMF) are the two major furan aldehyde inhibitors generated from lignocellulose dilute acid pretreatment which significantly inhibit subsequent microbial cell growth and ethanol fermentation. is an important strain for cellulosic ethanol fermentation but can be severely inhibited by furfural and (or) HMF. Previous study showed that contains its native oxidoreductases to catalyze the conversion of furfural and HMF, but the corresponding genes have not been identified.

Results: This study identified a NADPH-dependent alcohol dehydrogenase gene ZMO1771 from ZM4, which is responsible for the efficient reduction of furfural and HMF. Over-expression of ZMO1771 in significantly increased the conversion rate to both furfural and HMF and resulted in an accelerated cell growth and improved ethanol productivity in corn stover hydrolysate. Further, the ethanol fermentation performance was enhanced again by co-expression of the transhydrogenase gene with ZMO1771 by elevating the NADPH availability.

Conclusions: A genetically modified by co-expressing alcohol dehydrogenase gene ZMO1771 with transhydrogenase gene showed enhanced conversion rate of furfural and HMF and accelerated ethanol fermentability from lignocellulosic hydrolysate. The results presented in this study provide an important method on constructing robust strains for efficient ethanol fermentation from lignocellulose feedstock.

Graphical Abstract:

Citing Articles

Production of cellulosic ethanol and value-added products from corn fiber.

Guo Y, Liu G, Ning Y, Li X, Hu S, Zhao J Bioresour Bioprocess. 2024; 9(1):81.

PMID: 38647596 PMC: 10991675. DOI: 10.1186/s40643-022-00573-9.

Mechanism of furfural toxicity and metabolic strategies to engineer tolerance in microbial strains.

Jilani S, Olson D Microb Cell Fact. 2023; 22(1):221.

PMID: 37891678 PMC: 10612203. DOI: 10.1186/s12934-023-02223-x.

Cold plasma pretreatment reinforces the lignocellulose-derived aldehyde inhibitors tolerance and bioethanol fermentability for Zymomonas mobilis.

Yi X, Yang D, Xu X, Wang Y, Guo Y, Zhang M Biotechnol Biofuels Bioprod. 2023; 16(1):102.

PMID: 37322470 PMC: 10273749. DOI: 10.1186/s13068-023-02354-8.

Unraveling the mechanism of furfural tolerance in engineered by genomics.

Zou L, Jin X, Tao Y, Zheng Z, Ouyang J Front Microbiol. 2022; 13:1035263.

PMID: 36338095 PMC: 9630843. DOI: 10.3389/fmicb.2022.1035263.

Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Strain F211.

Hu D, Wang Z, He M, Ma Y Front Microbiol. 2021; 12:736583.

PMID: 34858360 PMC: 8631904. DOI: 10.3389/fmicb.2021.736583.

References

Shui Z, Qin H, Wu B, Ruan Z, Wang L, Tan F . Adaptive laboratory evolution of ethanologenic Zymomonas mobilis strain tolerant to furfural and acetic acid inhibitors. Appl Microbiol Biotechnol. 2015; 99(13):5739-48. DOI: 10.1007/s00253-015-6616-z. View

Klinke H, Thomsen A, Ahring B . Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass. Appl Microbiol Biotechnol. 2004; 66(1):10-26. DOI: 10.1007/s00253-004-1642-2. View

Almeida J, Roder A, Modig T, Laadan B, Liden G, Gorwa-Grauslund M . NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2008; 78(6):939-45. DOI: 10.1007/s00253-008-1364-y. View

Agrawal M, Chen R . Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4. Biotechnol Lett. 2011; 33(11):2127-33. DOI: 10.1007/s10529-011-0677-6. View

Yang S, Fei Q, Zhang Y, Contreras L, Utturkar S, Brown S . Zymomonas mobilis as a model system for production of biofuels and biochemicals. Microb Biotechnol. 2016; 9(6):699-717. PMC: 5072187. DOI: 10.1111/1751-7915.12408. View

Jan J, Martinez I, Wang Y, Bennett G, San K . Metabolic engineering and transhydrogenase effects on NADPH availability in Escherichia coli. Biotechnol Prog. 2013; 29(5):1124-30. DOI: 10.1002/btpr.1765. View

Tan F, Dai L, Wu B, Qin H, Shui Z, Wang J . Improving furfural tolerance of Zymomonas mobilis by rewiring a sigma factor RpoD protein. Appl Microbiol Biotechnol. 2015; 99(12):5363-71. DOI: 10.1007/s00253-015-6577-2. View

Ishii J, Yoshimura K, Hasunuma T, Kondo A . Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway. Appl Microbiol Biotechnol. 2012; 97(6):2597-607. DOI: 10.1007/s00253-012-4376-6. View

Taylor M, Mulako I, Tuffin M, Cowan D . Understanding physiological responses to pre-treatment inhibitors in ethanologenic fermentations. Biotechnol J. 2012; 7(9):1169-81. DOI: 10.1002/biot.201100335. View

10.

Franden M, Pilath H, Mohagheghi A, Pienkos P, Zhang M . Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates. Biotechnol Biofuels. 2013; 6(1):99. PMC: 3716709. DOI: 10.1186/1754-6834-6-99. View

11.

Liu Z, Moon J, Andersh B, Slininger P, Weber S . Multiple gene-mediated NAD(P)H-dependent aldehyde reduction is a mechanism of in situ detoxification of furfural and 5-hydroxymethylfurfural by Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2008; 81(4):743-53. DOI: 10.1007/s00253-008-1702-0. View

12.

Yang S, Pelletier D, Lu T, Brown S . The Zymomonas mobilis regulator hfq contributes to tolerance against multiple lignocellulosic pretreatment inhibitors. BMC Microbiol. 2010; 10:135. PMC: 2877685. DOI: 10.1186/1471-2180-10-135. View

13.

Mussatto S, Roberto I . Alternatives for detoxification of diluted-acid lignocellulosic hydrolyzates for use in fermentative processes: a review. Bioresour Technol. 2004; 93(1):1-10. DOI: 10.1016/j.biortech.2003.10.005. View

14.

Sanchez A, Andrews J, Hussein I, Bennett G, San K . Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly(3-hydroxybutyrate) in Escherichia coli. Biotechnol Prog. 2006; 22(2):420-5. DOI: 10.1021/bp050375u. View

15.

Liu Z, Slininger P, Dien B, Berhow M, Kurtzman C, Gorsich S . Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2,5-bis-hydroxymethylfuran. J Ind Microbiol Biotechnol. 2004; 31(8):345-52. DOI: 10.1007/s10295-004-0148-3. View

16.

Miller E, Jarboe L, Yomano L, York S, Shanmugam K, Ingram L . Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coli. Appl Environ Microbiol. 2009; 75(13):4315-23. PMC: 2704836. DOI: 10.1128/AEM.00567-09. View

17.

Petersson A, Almeida J, Modig T, Karhumaa K, Hahn-Hagerdal B, Gorwa-Grauslund M . A 5-hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance. Yeast. 2006; 23(6):455-64. DOI: 10.1002/yea.1370. View

18.

Wang X, Miller E, Yomano L, Zhang X, Shanmugam K, Ingram L . Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate. Appl Environ Microbiol. 2011; 77(15):5132-40. PMC: 3147458. DOI: 10.1128/AEM.05008-11. View

19.

Hasunuma T, Ismail K, Nambu Y, Kondo A . Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural. J Biosci Bioeng. 2013; 117(2):165-169. DOI: 10.1016/j.jbiosc.2013.07.007. View

20.

Zhang M, Eddy C, Deanda K, Finkelstein M, Picataggio S . Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis. Science. 1995; 267(5195):240-3. DOI: 10.1126/science.267.5195.240. View