Growth on Alpha-Ketoglutarate Increases Oxidative Stress Resistance in the Yeast

Overview

Journal Int J Microbiol

Publisher Wiley

Specialty Microbiology

Date 2017 Feb 4

PMID 28154578

Citations 11

Authors

Maria Bayliak

Nadia Burdyliuk

Volodymyr Lushchak

Affiliations

Soon will be listed here.

Abstract

Alpha-ketoglutarate (AKG) is an important intermediate in cell metabolism, linking anabolic and catabolic processes. The effect of exogenous AKG on stress resistance in cells was studied. The growth on AKG increased resistance of yeast cells to stresses, but the effects depended on AKG concentration and type of stressor. Wild-type yeast cells grown on AKG were more resistant to hydrogen peroxide, menadione, and transition metal ions (Fe and Cu) but not to ethanol and heat stress as compared with control ones. Deficiency in SODs or catalases abolished stress-protective effects of AKG. AKG-supplemented growth led to higher values of total metabolic activity, level of low-molecular mass thiols, and activities of catalase and glutathione reductase in wild-type cells compared with the control. The results suggest that exogenous AKG may enhance cell metabolism leading to induction of mild oxidative stress. It turn, it results in activation of antioxidant system that increases resistance of cells to HO and other stresses. The presence of genes encoding SODs or catalases is required for the expression of protective effects of AKG.

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