New Insights into the Function of Rab GTPases in the Context of Exosomal Secretion

Overview

Journal Small GTPases

Specialties Biochemistry
Cell Biology

Date 2017 Feb 1

PMID 28135905

Citations 160

Authors

Lionel Blanc

Michel Vidal

Affiliations

Soon will be listed here.

Abstract

In the last two decades, extracellular vesicle-mediated communication between cells has become a major field in cell biology. However, the function of extracellular vesicles is far from clear, especially due to the disparity of released vesicles by cells. Basically, one must consider vesicles budding from the cell plasma membrane (ectosomes) and vesicles released upon fusion of an endosomal multivesicular compartment (exosomes). Moreover, even for exosomes, we report and discuss here the possibility that different routes regulated by specific Rab GTPases might produce exosomes having various biologic functions.

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References

Zhang X, Ellis S, Sriratana A, Mitchell C, Rowe T . Sec15 is an effector for the Rab11 GTPase in mammalian cells. J Biol Chem. 2004; 279(41):43027-34. DOI: 10.1074/jbc.M402264200. View

Zerial M, McBride H . Rab proteins as membrane organizers. Nat Rev Mol Cell Biol. 2001; 2(2):107-17. DOI: 10.1038/35052055. View

Booth A, Fang Y, Fallon J, Yang J, Hildreth J, Gould S . Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane. J Cell Biol. 2006; 172(6):923-35. PMC: 2063735. DOI: 10.1083/jcb.200508014. View

Golachowska M, Hoekstra D, van Ijzendoorn S . Recycling endosomes in apical plasma membrane domain formation and epithelial cell polarity. Trends Cell Biol. 2010; 20(10):618-26. DOI: 10.1016/j.tcb.2010.08.004. View

Aguzzi A, Lakkaraju A . Cell Biology of Prions and Prionoids: A Status Report. Trends Cell Biol. 2015; 26(1):40-51. DOI: 10.1016/j.tcb.2015.08.007. View

Cauvin C, Rosendale M, Gupta-Rossi N, Rocancourt M, Larraufie P, Salomon R . Rab35 GTPase Triggers Switch-like Recruitment of the Lowe Syndrome Lipid Phosphatase OCRL on Newborn Endosomes. Curr Biol. 2016; 26(1):120-8. DOI: 10.1016/j.cub.2015.11.040. View

Johnstone R, Adam M, Hammond J, Orr L, Turbide C . Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). J Biol Chem. 1987; 262(19):9412-20. View

Denzer K, Kleijmeer M, Heijnen H, Stoorvogel W, Geuze H . Exosome: from internal vesicle of the multivesicular body to intercellular signaling device. J Cell Sci. 2000; 113 Pt 19:3365-74. DOI: 10.1242/jcs.113.19.3365. View

Blanc L, Barres C, Bette-Bobillo P, Vidal M . Reticulocyte-secreted exosomes bind natural IgM antibodies: involvement of a ROS-activatable endosomal phospholipase iPLA2. Blood. 2007; 110(9):3407-16. DOI: 10.1182/blood-2007-04-085845. View

10.

Yu X, Prekeris R, Gould G . Role of endosomal Rab GTPases in cytokinesis. Eur J Cell Biol. 2006; 86(1):25-35. DOI: 10.1016/j.ejcb.2006.10.002. View

11.

de Gassart A, Geminard C, Hoekstra D, Vidal M . Exosome secretion: the art of reutilizing nonrecycled proteins?. Traffic. 2004; 5(11):896-903. DOI: 10.1111/j.1600-0854.2004.00223.x. View

12.

Kouranti I, Sachse M, Arouche N, Goud B, Echard A . Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis. Curr Biol. 2006; 16(17):1719-25. DOI: 10.1016/j.cub.2006.07.020. View

13.

Ramel D, Wang X, Laflamme C, Montell D, Emery G . Rab11 regulates cell-cell communication during collective cell movements. Nat Cell Biol. 2013; 15(3):317-24. PMC: 4006229. DOI: 10.1038/ncb2681. View

14.

Lai F, Stubbs L, Artzt K . Molecular analysis of mouse Rab11b: a new type of mammalian YPT/Rab protein. Genomics. 1994; 22(3):610-6. DOI: 10.1006/geno.1994.1434. View

15.

Koles K, Nunnari J, Korkut C, Barria R, Brewer C, Li Y . Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons. J Biol Chem. 2012; 287(20):16820-34. PMC: 3351299. DOI: 10.1074/jbc.M112.342667. View

16.

Pan B, Teng K, Wu C, Adam M, Johnstone R . Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes. J Cell Biol. 1985; 101(3):942-8. PMC: 2113705. DOI: 10.1083/jcb.101.3.942. View

17.

Fitzner D, Schnaars M, van Rossum D, Krishnamoorthy G, Dibaj P, Bakhti M . Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis. J Cell Sci. 2011; 124(Pt 3):447-58. DOI: 10.1242/jcs.074088. View

18.

Baietti M, Zhang Z, Mortier E, Melchior A, Degeest G, Geeraerts A . Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012; 14(7):677-85. DOI: 10.1038/ncb2502. View

19.

Bobrie A, Krumeich S, Reyal F, Recchi C, Moita L, Seabra M . Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012; 72(19):4920-30. DOI: 10.1158/0008-5472.CAN-12-0925. View

20.

DSouza-Schorey C, Clancy J . Tumor-derived microvesicles: shedding light on novel microenvironment modulators and prospective cancer biomarkers. Genes Dev. 2012; 26(12):1287-99. PMC: 3387656. DOI: 10.1101/gad.192351.112. View