Application of the PAMONO-Sensor for Quantiﬁcation of Microvesicles and Determination of Nano-Particle Size Distribution

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2017 Jan 31

PMID 28134825

Citations 10

Authors

Victoria Shpacovitch

Irina Sidorenko

Jan Eric Lenssen

Vladimir Temchura

Frank Weichert

Heinrich Muller

Klaus Uberla

Alexander Zybin

Alexander Schramm

Roland Hergenroder

Affiliations

Soon will be listed here.

Abstract

The PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles-microvesicles (100-1000 nm)-also attracts growing interest as biomarkers of different pathologies and needs development of novel techniques for characterization. This work shows the applicability of a PAMONO-sensor for selective detection of microvesicles in aquatic samples. The sensor permits comparison of relative concentrations of microvesicles between samples. We also study a possibility of repeated use of a sensor chip after elution of the microvesicle capturing layer. Moreover, we improve the detection features of the PAMONO-sensor. The detection process utilizes novel machine learning techniques on the sensor image data to estimate particle size distributions of nano-particles in polydisperse samples. Altogether, our ﬁndings expand analytical features and the application ﬁeld of the PAMONO-sensor. They can also serve for a maturation of diagnostic tools based on the PAMONO-sensor platform.

Citing Articles

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Al-Bataineh Q, Telfah A, Tavares C, Hergenroder R Sci Rep. 2023; 13(1):22405.

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Switchable Polyacrylic Acid Polyelectrolyte Brushes for Surface Plasmon Resonance Applications.

Al-Bataineh Q, Telfah A, Shpacovitch V, Tavares C, Hergenroder R Sensors (Basel). 2023; 23(9).

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Surface Plasmon Resonance Sensitivity Enhancement Based on Protonated Polyaniline Films Doped by Aluminum Nitrate.

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PMID: 36551089 PMC: 9775065. DOI: 10.3390/bios12121122.

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