The Paranodal Cytoskeleton Clusters Na Channels at Nodes of Ranvier

Overview

Journal Elife

Specialty Biology

Date 2017 Jan 31

PMID 28134616

Citations 45

Authors

Veronique Amor

Chuansheng Zhang

Anna Vainshtein

Ao Zhang

Daniel R Zollinger

Yael Eshed-Eisenbach

Peter J Brophy

Matthew N Rasband

Elior Peles

Affiliations

Soon will be listed here.

Abstract

A high density of Na channels at nodes of Ranvier is necessary for rapid and efficient action potential propagation in myelinated axons. Na+ channel clustering is thought to depend on two axonal cell adhesion molecules that mediate interactions between the axon and myelinating glia at the nodal gap (i.e., NF186) and the paranodal junction (i.e., Caspr). Here we show that while Na channels cluster at nodes in the absence of NF186, they fail to do so in double conditional knockout mice lacking both NF186 and the paranodal cell adhesion molecule Caspr, demonstrating that a paranodal junction-dependent mechanism can cluster Na channels at nodes. Furthermore, we show that paranode-dependent clustering of nodal Na channels requires axonal βII spectrin which is concentrated at paranodes. Our results reveal that the paranodal junction-dependent mechanism of Nachannel clustering is mediated by the spectrin-based paranodal axonal cytoskeleton.

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