Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2017 Jan 28

PMID 28129503

Citations 204

Authors

Rubul Mout

Moumita Ray

Gulen Yesilbag Tonga

Yi-Wei Lee

Tristan Tay

Kanae Sasaki

Vincent M Rotello

Affiliations

Soon will be listed here.

Abstract

Genome editing through the delivery of CRISPR/Cas9-ribonucleoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here, we report a remarkably highly efficient (∼90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the coengineering of Cas9 protein and carrier nanoparticles. This construct provides effective (∼30%) gene editing efficiency and opens up opportunities in studying genome dynamics.

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