Anticancer Effects of the Marine Sponge Sp. Extract on Wild-Type and P53 Knockout HCT116 Cells

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2017 Jan 28

PMID 28127380

Citations 4

Authors

Kiheon Choi

Hyun Kyung Lim

Sung Ryong Oh

Woo-Hyun Chung

Joohee Jung

Affiliations

Soon will be listed here.

Abstract

Interest in marine bioresources is increasing in the drug development sector. In particular, marine sponges produce a wide range of unique metabolites that enable them to survive in challenging environments, which makes them attractive sources of candidate pharmaceuticals. In previous study, we investigated over 40 marine specimens collected in Micronesia and provided by the Korean Institute of Ocean Science and Technology, for their antiproliferative effects on various cancer cell lines, and sp. extract (LSSE) was found to have a marked antiproliferative effect. In the present study, we investigated the mechanism responsible for its anticancer effect on wild-type p53 (WT) or p53 knockout (KO) HCT116 cells. LSSE inhibited cell viability and induced apoptotic cell death more so in HCT116 p53 KO cells than the WT. HCT116 WT cells treated with LSSE underwent apoptosis associated with the induction of p53 and its target genes. On the other hand, in HCT116 p53 KO cells, LSSE reduced mTOR and Bcl-2 and increased Beclin-1 and LC3-II protein levels, suggesting autophagy induction. These results indicate that the mechanisms responsible for the anticancer effect of LSSE depend on p53 status.

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