Continuous Cell Supply from Krt7-expressing Hematopoietic Stem Cells During Native Hematopoiesis Revealed by Targeted in Vivo Gene Transfer Method

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jan 19

PMID 28098173

Citations 10

Authors

Yoko Tajima

Keiichi Ito

Ayumi Umino

Adam C Wilkinson

Hiromitsu Nakauchi

Satoshi Yamazaki

Affiliations

Soon will be listed here.

Abstract

The nature of hematopoietic stem cells under normal hematopoiesis remained largely unknown due to the limited assays available to monitor their behavior in situ. Here, we develop a new mouse model to transfer genes specifically into the primitive hematopoietic stem cell compartment through the utilization of a modified Rcas/TVA system. We succeeded in transferring a GFP reporter gene into adult hematopoietic stem cells in vivo, which are predominantly quiescent, by generating pseudotyped-lentivirus. Furthermore, we demonstrate the utility of this system to study neonatal hematopoiesis, a developmental stage that has been difficult to analyze to date. Using the system developed in this study, we observed continuous multi-lineage hematopoietic cell supply in peripheral blood from Krt7-positive hematopoietic stem cells during unperturbed homeostatic condition. This powerful experimental system could provide a new standard tool to analyze hematopoiesis under physiological condition without transplantation.

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