Identification and Monitoring of Korean Medicines Derived from Spp. by Using ITS and DNA Marker

In this study, we identified and evaluated the genetic relationships among plants, which are used in traditional medicine. We also attempted to monitor the distribution of traditional medicines derived from by using DNA barcoding and a species-specific DNA marker. Plants of the genus , and in particular , are commonly used as medicinal herbs in the form of Cinnamomi Ramulus, Cinnamomi Cortex, and Cassiae Cortex Interior. However, it is difficult to distinguish among different species based on morphological features, and so to overcome this limitation, nucleotide sequences of the internal transcribed spacer (ITS) region of DNA were determined and compared. On the basis of the discrepancy in determined ITS sequences, a 408-bp product, amplified by the primer pair CC F1/CC R3, was developed as a -specific DNA marker. Using the developed DNA marker in combination with the ITS 2 nucleotide sequence, we monitored imported and commercially supplied medicinal products derived from plants in markets in Korean, China, and Japan. The results revealed that most of the specimens monitored were derived from .