LncRNA-LINC00152 Down-regulated by MiR-376c-3p Restricts Viability and Promotes Apoptosis of Colorectal Cancer Cells

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2017 Jan 13

PMID 28078002

Citations 50

Authors

Yu-Hao Zhang

Jun Fu

Zhi-Jin Zhang

Cui-Cui Ge

Yi Yi

Affiliations

Soon will be listed here.

Abstract

Recent studies have demonstrated that the crucial regulatory roles of long noncoding RNAs (lncRNAs) in tumorigenesis. Expression levels of several lncRNAs are abnormally up-regulated or down-regulated and play a primary role in colorectal cancer (CRC) cell tumorigenesis. However, the potential role and regulatory mechanisms of the novel human lncRNA, LINC00152, in CRC cells are poorly understood. Here, we found that LINC00152 expression was significantly decreased in CRC tissues and CRC cell lines, and this change was more frequent in patients with advanced stage (tumor-node-metastasisi (TNM) III and IV). Overexpression of LINC00152 (LINC000152) resulted in decreased cell viability and increased apoptosis in CSC cell lines (HT-29 and SW480). Furthermore, decreased Ki-67 and B-cell lymphoma-2 (Bcl-2), and increased Fas, were observed in CSC cells. However, a change in Bax expression was undetected. Interestingly, microRNA (miR)-376c-3p down-regulated the expression of LINC00152 in CSC cells. Overexpression of miR-376c-3p (miR-376c-3p) enhanced viability and limited apoptosis of CSC cells. In addition, miR-376c-3p suppressed the effect of LINC00152 on the viability and apoptosis of CSC cells. Taken together, these data indicate that LINC00152 in CSC cells negatively regulated by miR-376c-3p, restricts cell viability and stimulates cell apoptosis, possibly by modulating the expression of Ki-67, Bcl-2, and Fas. MiR-376c-3p/LINC00152 plays an important role in the pathogenesis of CRC and may serve as a potential target for its diagnosis and treatment.

Citing Articles

Cross-species conserved miRNA as biomarker of radiation injury over a wide dose range using nonhuman primate model.

Chakraborty N, Dimitrov G, Kanan S, Lawrence A, Moyler C, Gautam A PLoS One. 2024; 19(11):e0311379.

PMID: 39570918 PMC: 11581275. DOI: 10.1371/journal.pone.0311379.

Long noncoding RNA KCNMA1-AS2 regulates the function of colorectal cancer cells and sponges miR-1227-5p.

Miao X, Wang F, Yunus M, Ismail I, Wang T BMC Cancer. 2024; 24(1):857.

PMID: 39026221 PMC: 11256649. DOI: 10.1186/s12885-024-12608-9.

Interaction of lncRNAs with mTOR in colorectal cancer: a systematic review.

Moslehian M, Shabkhizan R, Asadi M, Bazmani A, Mahdipour M, Haiaty S BMC Cancer. 2023; 23(1):512.

PMID: 37280524 PMC: 10243085. DOI: 10.1186/s12885-023-11008-9.

lncRNA cytoskeleton regulator RNA (CYTOR): Diverse functions in metabolism, inflammation and tumorigenesis, and potential applications in precision oncology.

Ou C, He X, Liu Y, Zhang X Genes Dis. 2023; 10(2):415-429.

PMID: 37223495 PMC: 10201560. DOI: 10.1016/j.gendis.2021.08.012.

Apoptosis evasion via long non-coding RNAs in colorectal cancer.

Irfan M, Javed Z, Khan K, Khan N, Docea A, Calina D Cancer Cell Int. 2022; 22(1):280.

PMID: 36076273 PMC: 9461221. DOI: 10.1186/s12935-022-02695-8.

References

Jiang W, Tian Y, Jiang S, Liu S, Zhao X, Tian D . MicroRNA-376c suppresses non-small-cell lung cancer cell growth and invasion by targeting LRH-1-mediated Wnt signaling pathway. Biochem Biophys Res Commun. 2016; 473(4):980-986. DOI: 10.1016/j.bbrc.2016.04.002. View

Edwards B, Noone A, Mariotto A, Simard E, Boscoe F, Henley S . Annual Report to the Nation on the status of cancer, 1975-2010, featuring prevalence of comorbidity and impact on survival among persons with lung, colorectal, breast, or prostate cancer. Cancer. 2013; 120(9):1290-314. PMC: 3999205. DOI: 10.1002/cncr.28509. View

Fu G, Ye G, Nadeem L, Ji L, Manchanda T, Wang Y . MicroRNA-376c impairs transforming growth factor-β and nodal signaling to promote trophoblast cell proliferation and invasion. Hypertension. 2013; 61(4):864-72. DOI: 10.1161/HYPERTENSIONAHA.111.203489. View

Butler A, Webb W, Lubin F . Regulatory RNAs and control of epigenetic mechanisms: expectations for cognition and cognitive dysfunction. Epigenomics. 2015; 8(1):135-51. PMC: 5514977. DOI: 10.2217/epi.15.79. View

Yin D, He X, Zhang E, Kong R, De W, Zhang Z . Long noncoding RNA GAS5 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer. Med Oncol. 2014; 31(11):253. DOI: 10.1007/s12032-014-0253-8. View

Wang K, Chang H . Molecular mechanisms of long noncoding RNAs. Mol Cell. 2011; 43(6):904-14. PMC: 3199020. DOI: 10.1016/j.molcel.2011.08.018. View

Dixon-McIver A, East P, Mein C, Cazier J, Molloy G, Chaplin T . Distinctive patterns of microRNA expression associated with karyotype in acute myeloid leukaemia. PLoS One. 2008; 3(5):e2141. PMC: 2373886. DOI: 10.1371/journal.pone.0002141. View

Seitz H, Royo H, Bortolin M, Lin S, Ferguson-Smith A, Cavaille J . A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain. Genome Res. 2004; 14(9):1741-8. PMC: 515320. DOI: 10.1101/gr.2743304. View

Li Q, Shao Y, Zhang X, Zheng T, Miao M, Qin L . Plasma long noncoding RNA protected by exosomes as a potential stable biomarker for gastric cancer. Tumour Biol. 2014; 36(3):2007-12. DOI: 10.1007/s13277-014-2807-y. View

10.

Karsa L, Lignini T, Patnick J, Lambert R, Sauvaget C . The dimensions of the CRC problem. Best Pract Res Clin Gastroenterol. 2010; 24(4):381-96. DOI: 10.1016/j.bpg.2010.06.004. View

11.

Xia T, Liao Q, Jiang X, Shao Y, Xiao B, Xi Y . Long noncoding RNA associated-competing endogenous RNAs in gastric cancer. Sci Rep. 2014; 4:6088. PMC: 4133709. DOI: 10.1038/srep06088. View

12.

Qiu J, Yan J . Long non-coding RNA LINC01296 is a potential prognostic biomarker in patients with colorectal cancer. Tumour Biol. 2015; 36(9):7175-83. DOI: 10.1007/s13277-015-3448-5. View

13.

Gajate C, Mollinedo F . Lipid raft-mediated Fas/CD95 apoptotic signaling in leukemic cells and normal leukocytes and therapeutic implications. J Leukoc Biol. 2015; 98(5):739-59. DOI: 10.1189/jlb.2MR0215-055R. View

14.

Agrawal R, Pandey P, Jha P, Dwivedi V, Sarkar C, Kulshreshtha R . Hypoxic signature of microRNAs in glioblastoma: insights from small RNA deep sequencing. BMC Genomics. 2014; 15:686. PMC: 4148931. DOI: 10.1186/1471-2164-15-686. View

15.

Wu Y, Tan C, Weng W, Deng Y, Zhang Q, Yang X . Long non-coding RNA Linc00152 is a positive prognostic factor for and demonstrates malignant biological behavior in clear cell renal cell carcinoma. Am J Cancer Res. 2016; 6(2):285-99. PMC: 4859660. View

16.

Thorenoor N, Faltejskova-Vychytilova P, Hombach S, Mlcochova J, Kretz M, Svoboda M . Long non-coding RNA ZFAS1 interacts with CDK1 and is involved in p53-dependent cell cycle control and apoptosis in colorectal cancer. Oncotarget. 2015; 7(1):622-37. PMC: 4808022. DOI: 10.18632/oncotarget.5807. View

17.

Sana J, Faltejskova P, Svoboda M, Slaby O . Novel classes of non-coding RNAs and cancer. J Transl Med. 2012; 10:103. PMC: 3434024. DOI: 10.1186/1479-5876-10-103. View

18.

Zhou J, Zhi X, Wang L, Wang W, Li Z, Tang J . Linc00152 promotes proliferation in gastric cancer through the EGFR-dependent pathway. J Exp Clin Cancer Res. 2015; 34:135. PMC: 4632266. DOI: 10.1186/s13046-015-0250-6. View

19.

Witalison E, Cui X, Causey C, Thompson P, Hofseth L . Molecular targeting of protein arginine deiminases to suppress colitis and prevent colon cancer. Oncotarget. 2015; 6(34):36053-62. PMC: 4742161. DOI: 10.18632/oncotarget.5937. View

20.

Hardwick J, Soane L . Multiple functions of BCL-2 family proteins. Cold Spring Harb Perspect Biol. 2013; 5(2). PMC: 3552500. DOI: 10.1101/cshperspect.a008722. View