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Regulating Chromosomal Movement by the Cochaperone FKB-6 Ensures Timely Pairing and Synapsis

Overview
Journal J Cell Biol
Specialty Cell Biology
Date 2017 Jan 13
PMID 28077446
Citations 8
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Abstract

In meiotic prophase I, homologous chromosome pairing is promoted through chromosome movement mediated by nuclear envelope proteins, microtubules, and dynein. After proper homologue pairing has been established, the synaptonemal complex (SC) assembles along the paired homologues, stabilizing their interaction and allowing for crossing over to occur. Previous studies have shown that perturbing chromosome movement leads to pairing defects and SC polycomplex formation. We show that FKB-6 plays a role in SC assembly and is required for timely pairing and proper double-strand break repair kinetics. FKB-6 localizes outside the nucleus, and in its absence, the microtubule network is altered. FKB-6 is required for proper movement of dynein, increasing resting time between movements. Attenuating chromosomal movement in fkb-6 mutants partially restores the defects in synapsis, in agreement with FKB-6 acting by decreasing chromosomal movement. Therefore, we suggest that FKB-6 plays a role in regulating dynein movement by preventing excess chromosome movement, which is essential for proper SC assembly and homologous chromosome pairing.

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References
1.
Niwa O, Shimanuki M, Miki F . Telomere-led bouquet formation facilitates homologous chromosome pairing and restricts ectopic interaction in fission yeast meiosis. EMBO J. 2000; 19(14):3831-40. PMC: 313979. DOI: 10.1093/emboj/19.14.3831. View

2.
Couteau F, Zetka M . HTP-1 coordinates synaptonemal complex assembly with homolog alignment during meiosis in C. elegans. Genes Dev. 2005; 19(22):2744-56. PMC: 1283966. DOI: 10.1101/gad.1348205. View

3.
Horn H, Kim D, Wright G, Wong E, Stewart C, Burke B . A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton. J Cell Biol. 2013; 202(7):1023-39. PMC: 3787381. DOI: 10.1083/jcb.201304004. View

4.
Erlejman A, Lagadari M, Harris D, Cox M, Galigniana M . Molecular chaperone activity and biological regulatory actions of the TPR-domain immunophilins FKBP51 and FKBP52. Curr Protein Pept Sci. 2014; 15(3):205-15. DOI: 10.2174/1389203715666140331113753. View

5.
Phillips C, Wong C, Bhalla N, Carlton P, Weiser P, Meneely P . HIM-8 binds to the X chromosome pairing center and mediates chromosome-specific meiotic synapsis. Cell. 2005; 123(6):1051-63. PMC: 4435792. DOI: 10.1016/j.cell.2005.09.035. View