The E3 Ubiquitin Ligase RNF114 and TAB1 Degradation Are Required for Maternal-to-zygotic Transition

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2017 Jan 12

PMID 28073917

Citations 32

Authors

Ye Yang

Cheng Zhou

Ying Wang

Weixiao Liu

Chao Liu

Liying Wang

Yujiao Liu

Yongliang Shang

Mingrui Li

Shuai Zhou

Yuanting Wang

Wentao Zeng

Jianli Zhou

Ran Huo

Wei Li

Affiliations

Soon will be listed here.

Abstract

The functional role of the ubiquitin-proteasome pathway during maternal-to-zygotic transition (MZT) remains to be elucidated. Here we show that the E3 ubiquitin ligase, Rnf114, is highly expressed in mouse oocytes and that knockdown of Rnf114 inhibits development beyond the two-cell stage. To study the underlying mechanism, we identify its candidate substrates using a 9,000-protein microarray and validate them using an in vitro ubiquitination system. We show that five substrates could be degraded by RNF114-mediated ubiquitination, including TAB1. Furthermore, the degradation of TAB1 in mouse early embryos is required for MZT, most likely because it activates the NF-κB pathway. Taken together, our study uncovers that RNF114-mediated ubiquitination and degradation of TAB1 activate the NF-κB pathway during MZT, and thus directly link maternal clearance to early embryo development.

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