Anti-viral Role of Toll Like Receptor 4 in Hepatitis B Virus Infection: An Study

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2017 Jan 7

PMID 28058014

Citations 3

Authors

Dipanwita Das

Neelakshi Sarkar

Isha Sengupta

Ananya Pal

Debraj Saha

Manikankana Bandopadhyay

Chandrima Das

Jimmy Narayan

Shivram Prasad Singh

Runu Chakravarty

Affiliations

Soon will be listed here.

Abstract

Aim: Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4 (TLR4) in hepatitis B virus (HBV) infection.

Methods: Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the HepG2.2.15 cells with TLR4 specific ligand. Nuclear factor (NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.

Results: The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.

Conclusion: The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future.

Citing Articles

Novel roles of lipopolysaccharide and TLR4/NF-κB signaling pathway in inflammatory response to liver injury in Budd-Chiari syndrome.

Li J, Chen X, Zhou C, Fang W, Lv W, Cheng D World J Gastrointest Surg. 2021; 13(11):1448-1462.

PMID: 34950433 PMC: 8649562. DOI: 10.4240/wjgs.v13.i11.1448.

Host Innate Immunity Against Hepatitis Viruses and Viral Immune Evasion.

Xu C, Chen J, Chen X Front Microbiol. 2021; 12:740464.

PMID: 34803956 PMC: 8598044. DOI: 10.3389/fmicb.2021.740464.

Multi-Omic Data Integration Allows Baseline Immune Signatures to Predict Hepatitis B Vaccine Response in a Small Cohort.

Shannon C, Blimkie T, Ben-Othman R, Gladish N, Amenyogbe N, Drissler S Front Immunol. 2020; 11:578801.

PMID: 33329547 PMC: 7734088. DOI: 10.3389/fimmu.2020.578801.

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