FeO Nanoparticles on Graphene Oxide Sheets for Isolation and Ultrasensitive Amperometric Detection of Cancer Biomarker Proteins

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2017 Jan 6

PMID 28056439

Citations 34

Authors

Mohamed Sharafeldin

Gregory W Bishop

Snehasis Bhakta

Abdelhamid El-Sawy

Steven L Suib

James F Rusling

Affiliations

Soon will be listed here.

Abstract

Ultrasensitive mediator-free electrochemical detection for biomarker proteins was achieved at low cost using a novel composite of FeO nanoparticles loaded onto graphene oxide (GO) nano-sheets (FeO@GO). This paramagnetic FeO@GO composite (1µm size range) was decorated with antibodies against prostate specific antigen (PSA) and prostate specific membrane antigen (PSMA), and then used to first capture these biomarkers and then deliver them to an 8-sensor detection chamber of a microfluidic immunoarray. Screen-printed carbon sensors coated with electrochemically reduced graphene oxide (ERGO) and a second set of antibodies selectively capture the biomarker-laden FeO@GO particles, which subsequently catalyze hydrogen peroxide reduction to detect PSA and PSMA. Accuracy was confirmed by good correlation between patient serum assays and enzyme-linked immuno-sorbent assays (ELISA). Excellent detection limits (LOD) of 15 fg/mL for PSA and 4.8 fg/mL for PSMA were achieved in serum. The LOD for PSA was 1000-fold better than the only previous report of PSA detection using FeO. Dynamic ranges were easily tunable for concentration ranges encountered in serum samples by adjusting the FeO@GO Concentration. Reagent cost was only $0.85 for a single 2-protein assay.

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