Proteomic Profiling of Neuroblastoma Cells Adhesion on Hyaluronic Acid-Based Surface for Neural Tissue Engineering

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2017 Jan 6

PMID 28053978

Citations 4

Authors

Ming-Hui Yang

Ko-Chin Chen

Pei-Wen Chiang

Tze-Wen Chung

Wan-Jou Chen

Pei-Yu Chu

Sharon Chia-Ju Chen

Yi-Shan Lu

Cheng-Hui Yuan

Ming-Chen Wang

Chia-Yang Lin

Ying-Fong Huang

Shiang-Bin Jong

Po-Chiao Lin

Yu-Chang Tyan

Affiliations

Soon will be listed here.

Abstract

The microenvironment of neuron cells plays a crucial role in regulating neural development and regeneration. Hyaluronic acid (HA) biomaterial has been applied in a wide range of medical and biological fields and plays important roles in neural regeneration. PC12 cells have been reported to be capable of endogenous NGF synthesis and secretion. The purpose of this research was to assess the effect of HA biomaterial combining with PC12 cells conditioned media (PC12 CM) in neural regeneration. Using SH-SY5Y cells as an experimental model, we found that supporting with PC12 CM enhanced HA function in SH-SY5Y cell proliferation and adhesion. Through RP-nano-UPLC-ESI-MS/MS analyses, we identified increased expression of HSP60 and RanBP2 in SH-SY5Y cells grown on HA-modified surface with cotreatment of PC12 CM. Moreover, we also identified factors that were secreted from PC12 cells and may promote SH-SY5Y cell proliferation and adhesion. Here, we proposed a biomaterial surface enriched with neurotrophic factors for nerve regeneration application.

Citing Articles

3D cell culture model: From ground experiment to microgravity study.

Ma C, Duan X, Lei X Front Bioeng Biotechnol. 2023; 11:1136583.

PMID: 37034251 PMC: 10080128. DOI: 10.3389/fbioe.2023.1136583.

Hydrogels: Properties and Applications in Biomedicine.

Ho T, Chang C, Chan H, Chung T, Shu C, Chuang K Molecules. 2022; 27(9).

PMID: 35566251 PMC: 9104731. DOI: 10.3390/molecules27092902.

Bioactivated Oxidized Polyvinyl Alcohol towards Next-Generation Nerve Conduits Development.

Stocco E, Barbon S, Lamanna A, De Rose E, Zamuner A, Sandrin D Polymers (Basel). 2021; 13(19).

PMID: 34641183 PMC: 8512895. DOI: 10.3390/polym13193372.

Mechanism of Activating the Proprioceptive NT-3/TrkC Signalling Pathway by Reverse Intervention for the Anterior Cruciate Ligament-Hamstring Reflex Arc with Electroacupuncture.

Zhang L, Zeng Y, Qi J, Guan T, Zhou X, He Y Biomed Res Int. 2018; 2018:6348764.

PMID: 29581981 PMC: 5822800. DOI: 10.1155/2018/6348764.

References

Mei L, Xiong W . Neuregulin 1 in neural development, synaptic plasticity and schizophrenia. Nat Rev Neurosci. 2008; 9(6):437-52. PMC: 2682371. DOI: 10.1038/nrn2392. View

Cho K, Yi H, Yeh A, Tserentsoodol N, Cuadrado L, Searle K . Haploinsufficiency of RanBP2 is neuroprotective against light-elicited and age-dependent degeneration of photoreceptor neurons. Cell Death Differ. 2008; 16(2):287-97. PMC: 2626153. DOI: 10.1038/cdd.2008.153. View

Sullivan S, Barthel L, Largent B, Raymond P . A goldfish Notch-3 homologue is expressed in neurogenic regions of embryonic, adult, and regenerating brain and retina. Dev Genet. 1997; 20(3):208-23. DOI: 10.1002/(SICI)1520-6408(1997)20:3<208::AID-DVG4>3.0.CO;2-B. View

Yang M, Jong S, Lu C, Lin Y, Chiang P, Tyan Y . Assessing the responses of cellular proteins induced by hyaluronic acid-modified surfaces utilizing a mass spectrometry-based profiling system: over-expression of CD36, CD44, CDK9, and PP2A. Analyst. 2012; 137(21):4921-33. DOI: 10.1039/c2an35368g. View

Khaing Z, Milman B, Vanscoy J, Seidlits S, Grill R, Schmidt C . High molecular weight hyaluronic acid limits astrocyte activation and scar formation after spinal cord injury. J Neural Eng. 2011; 8(4):046033. DOI: 10.1088/1741-2560/8/4/046033. View

Rooney P, Kumar S, Ponting J, Wang M . The role of hyaluronan in tumour neovascularization (review). Int J Cancer. 1995; 60(5):632-6. DOI: 10.1002/ijc.2910600511. View

Jiang D, Liang J, Noble P . Hyaluronan in tissue injury and repair. Annu Rev Cell Dev Biol. 2007; 23:435-61. DOI: 10.1146/annurev.cellbio.23.090506.123337. View

Slevin M, Krupinski J, Kumar S, Gaffney J . Angiogenic oligosaccharides of hyaluronan induce protein tyrosine kinase activity in endothelial cells and activate a cytoplasmic signal transduction pathway resulting in proliferation. Lab Invest. 1998; 78(8):987-1003. View

Feng M, Zhang L, Liu Z, Zhou P, Lu X . The expression and release of Hsp60 in 6-OHDA induced in vivo and in vitro models of Parkinson's disease. Neurochem Res. 2013; 38(10):2180-9. DOI: 10.1007/s11064-013-1127-8. View

10.

Greene J, Sidle D . The Hyaluronic Acid Fillers: Current Understanding of the Tissue Device Interface. Facial Plast Surg Clin North Am. 2015; 23(4):423-32. DOI: 10.1016/j.fsc.2015.07.002. View

11.

Slevin M, Kumar S, Gaffney J . Angiogenic oligosaccharides of hyaluronan induce multiple signaling pathways affecting vascular endothelial cell mitogenic and wound healing responses. J Biol Chem. 2002; 277(43):41046-59. DOI: 10.1074/jbc.M109443200. View

12.

Marino Gammazza A, Colangeli R, Orban G, Pierucci M, Di Gennaro G, Lo Bello M . Hsp60 response in experimental and human temporal lobe epilepsy. Sci Rep. 2015; 5:9434. PMC: 4371150. DOI: 10.1038/srep09434. View

13.

Archibald S, Krarup C, Shefner J, Li S, Madison R . A collagen-based nerve guide conduit for peripheral nerve repair: an electrophysiological study of nerve regeneration in rodents and nonhuman primates. J Comp Neurol. 1991; 306(4):685-96. DOI: 10.1002/cne.903060410. View

14.

Richards M, Dahiyat B, Arm D, Brown P, Leong K . Evaluation of polyphosphates and polyphosphonates as degradable biomaterials. J Biomed Mater Res. 1991; 25(9):1151-67. DOI: 10.1002/jbm.820250908. View

15.

Castagnet P, Mavlyutov T, Cai Y, Zhong F, Ferreira P . RPGRIP1s with distinct neuronal localization and biochemical properties associate selectively with RanBP2 in amacrine neurons. Hum Mol Genet. 2003; 12(15):1847-63. DOI: 10.1093/hmg/ddg202. View

16.

Anton E, Ghashghaei H, Weber J, McCann C, Fischer T, Cheung I . Receptor tyrosine kinase ErbB4 modulates neuroblast migration and placement in the adult forebrain. Nat Neurosci. 2004; 7(12):1319-28. DOI: 10.1038/nn1345. View

17.

Lisignoli G, Cristino S, Piacentini A, Toneguzzi S, Grassi F, Cavallo C . Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold. Biomaterials. 2005; 26(28):5677-86. DOI: 10.1016/j.biomaterials.2005.02.031. View

18.

Rochkind S, Nevo Z . Recovery of peripheral nerve with massive loss defect by tissue engineered guiding regenerative gel. Biomed Res Int. 2014; 2014:327578. PMC: 4106053. DOI: 10.1155/2014/327578. View

19.

Pichler A, Gast A, Seeler J, Dejean A, Melchior F . The nucleoporin RanBP2 has SUMO1 E3 ligase activity. Cell. 2002; 108(1):109-20. DOI: 10.1016/s0092-8674(01)00633-x. View

20.

Gill J, Schenone A, Podratz J, Windebank A . Autocrine regulation of neurite outgrowth from PC12 cells by nerve growth factor. Brain Res Mol Brain Res. 1998; 57(1):123-31. DOI: 10.1016/s0169-328x(98)00080-1. View