A Novel System for Comparative Analyses of Bone Cells and Bacteria Under Electrical Stimulation

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2017 Jan 4

PMID 28044132

Citations 15

Authors

Thomas Josef Dauben

Josefin Ziebart

Thomas Bender

Sarah Zaatreh

Bernd Kreikemeyer

Rainer Bader

Affiliations

Soon will be listed here.

Abstract

Electrical stimulation is a promising approach to enhance bone regeneration while having potential to inhibit bacterial growth. To investigate effects of alternating electric field stimulation on both human osteoblasts and bacteria, a novel system was designed. Electric field distribution was simulated numerically and proved by experimental validation. Cells were stimulated on Ti6Al4V electrodes and in short distance to electrodes. Bacterial growth was enumerated in supernatant and on the electrode surface and biofilm formation was quantified. Electrical stimulation modulated gene expression of osteoblastic differentiation markers in a voltage-dependent manner, resulting in significantly enhanced osteocalcin mRNA synthesis rate on electrodes after stimulation with 1.4. While collagen type I synthesis increased when stimulated with 0.2, it decreased after stimulation with 1.4. Only slight and infrequent influence on bacterial growth was observed following stimulations with 0.2 and 1.4 after 48 and 72 h, respectively. In summary this novel test system is applicable for extended studies concerning definition of appropriate stimulation parameters for bone cell growth and differentiation, bacterial growth suppression, and investigation of general effects of electrical stimulation.

Citing Articles

Electrical stimulation for cartilage tissue engineering - A critical review from an engineer's perspective.

Zimmermann J, Farooqi A, van Rienen U Heliyon. 2024; 10(19):e38112.

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Combining Electrostimulation with Impedance Sensing to Promote and Track Osteogenesis within a Titanium Implant.

Engel N, Dau M, Engel V, Franz D, Klemmstein F, Thanisch C Biomedicines. 2023; 11(3).

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Pulsed Electrical Stimulation Affects Osteoblast Adhesion and Calcium Ion Signaling.

Staehlke S, Bielfeldt M, Zimmermann J, Gruening M, Barke I, Freitag T Cells. 2022; 11(17).

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Electrical stimulation to promote osseointegration of bone anchoring implants: a topical review.

Pettersen E, Anderson J, Ortiz-Catalan M J Neuroeng Rehabil. 2022; 19(1):31.

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Electronic Bone Growth Stimulators for Augmentation of Osteogenesis in and Models: A Narrative Review of Electrical Stimulation Mechanisms and Device Specifications.

Nicksic P, Donnelly D, Hesse M, Bedi S, Verma N, Seitz A Front Bioeng Biotechnol. 2022; 10:793945.

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