Primordial Germ Cell Differentiation of Nuclear Transfer Embryonic Stem Cells Using Surface Modified Electroconductive Scaffolds

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 2017 Jan 1

PMID 28039620

Authors

Tarlan Eslami-Arshaghi

Saeid Vakilian

Ehsan Seyedjafari

Abdolreza Ardeshirylajimi

Masoud Soleimani

Mohammad Salehi

Affiliations

Soon will be listed here.

Abstract

A combination of nanotopographical cues and surface modification of collagen and fibronectin is a potential platform in primordial germ cells (PGCs) differentiation. In the present study, the synergistic effect of nanotopography and surface modification on differentiation of nuclear transfer embryonic stem cells (nt-ESCs) toward PGC lineage was investigated. In order to achieve this goal, poly-anyline (PANi) was mix within poly-L-lactic acid (PLLA). Afterward, the random composite mats were fabricated using PLLA and PANi mix solution. The nanofiber topography notably upregulated the expressions of prdm14, mvh and c-kit compared with tissue culture polystyrene (TCP). Moreover, the combination of nanofiber topography and surface modification resulted in more enhancement of PGCs differentiation compared with non-modified nanofibrous scaffold. Additionally, gene expression results showed that mvh and c-kit were expressed at higher intensity in cells exposed to collagen and fibronectin rather than collagen or fibronectin solitary. These results demonstrated the importance of combined effect of collagen and fibronectin in order to develop a functional extracellular matrix (ECM) mimic in directing stem cell fate and the potential of such biofunctional scaffolds for treatment of infertility.

References

Stoop H, Honecker F, van de Geijn G, Gillis A, Cools M, de Boer M . Stem cell factor as a novel diagnostic marker for early malignant germ cells. J Pathol. 2008; 216(1):43-54. DOI: 10.1002/path.2378. View

Marcho C, Cui W, Mager J . Epigenetic dynamics during preimplantation development. Reproduction. 2015; 150(3):R109-20. PMC: 4529766. DOI: 10.1530/REP-15-0180. View

Yoo H, Kim T, Park T . Surface-functionalized electrospun nanofibers for tissue engineering and drug delivery. Adv Drug Deliv Rev. 2009; 61(12):1033-42. DOI: 10.1016/j.addr.2009.07.007. View

Clark A, Bodnar M, Fox M, Rodriquez R, Abeyta M, Firpo M . Spontaneous differentiation of germ cells from human embryonic stem cells in vitro. Hum Mol Genet. 2004; 13(7):727-39. DOI: 10.1093/hmg/ddh088. View

Kehler J, Tolkunova E, Koschorz B, Pesce M, Gentile L, Boiani M . Oct4 is required for primordial germ cell survival. EMBO Rep. 2004; 5(11):1078-83. PMC: 1299174. DOI: 10.1038/sj.embor.7400279. View

Toyooka Y, Tsunekawa N, Akasu R, Noce T . Embryonic stem cells can form germ cells in vitro. Proc Natl Acad Sci U S A. 2003; 100(20):11457-62. PMC: 208779. DOI: 10.1073/pnas.1932826100. View

Print C, Loveland K . Germ cell suicide: new insights into apoptosis during spermatogenesis. Bioessays. 2000; 22(5):423-30. DOI: 10.1002/(SICI)1521-1878(200005)22:5<423::AID-BIES4>3.0.CO;2-0. View

Bhartiya D, Hinduja I, Patel H, Bhilawadikar R . Making gametes from pluripotent stem cells--a promising role for very small embryonic-like stem cells. Reprod Biol Endocrinol. 2014; 12:114. PMC: 4255929. DOI: 10.1186/1477-7827-12-114. View

Sharma R, Biedenharn K, Fedor J, Agarwal A . Lifestyle factors and reproductive health: taking control of your fertility. Reprod Biol Endocrinol. 2013; 11:66. PMC: 3717046. DOI: 10.1186/1477-7827-11-66. View

10.

Mansouri V, Salehi M, Nourozian M, Fadaei F, Farahani R, Piryaei A . The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells. Genet Mol Biol. 2015; 38(2):220-6. PMC: 4530652. DOI: 10.1590/S1415-475738138120140213. View

11.

Laflamme M, Chen K, Naumova A, Muskheli V, Fugate J, Dupras S . Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat Biotechnol. 2007; 25(9):1015-24. DOI: 10.1038/nbt1327. View

12.

Ghasemi-Mobarakeh L, Prabhakaran M, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S . Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. World J Stem Cells. 2015; 7(4):728-44. PMC: 4444613. DOI: 10.4252/wjsc.v7.i4.728. View

13.

Prabhakaran M, Mobarakeh L, Kai D, Karbalaie K, Nasr-Esfahani M, Ramakrishna S . Differentiation of embryonic stem cells to cardiomyocytes on electrospun nanofibrous substrates. J Biomed Mater Res B Appl Biomater. 2013; 102(3):447-54. DOI: 10.1002/jbm.b.33022. View

14.

Nikolic A, Volarevic V, Armstrong L, Lako M, Stojkovic M . Primordial Germ Cells: Current Knowledge and Perspectives. Stem Cells Int. 2015; 2016:1741072. PMC: 4655300. DOI: 10.1155/2016/1741072. View

15.

Mintz B, RUSSELL E . Gene-induced embryological modifications of primordial germ cells in the mouse. J Exp Zool. 1957; 134(2):207-37. DOI: 10.1002/jez.1401340202. View

16.

Li W, Tuli R, Huang X, Laquerriere P, Tuan R . Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials. 2005; 26(25):5158-66. DOI: 10.1016/j.biomaterials.2005.01.002. View

17.

Saitou M . Specification of the germ cell lineage in mice. Front Biosci (Landmark Ed). 2009; 14(3):1068-87. DOI: 10.2741/3294. View

18.

Chuma S, Kanatsu-Shinohara M, Inoue K, Ogonuki N, Miki H, Toyokuni S . Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis. Development. 2004; 132(1):117-22. DOI: 10.1242/dev.01555. View

19.

Ohinata Y, Payer B, OCarroll D, Ancelin K, Ono Y, Sano M . Blimp1 is a critical determinant of the germ cell lineage in mice. Nature. 2005; 436(7048):207-13. DOI: 10.1038/nature03813. View

20.

Levenberg S, Huang N, Lavik E, Rogers A, Itskovitz-Eldor J, Langer R . Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. Proc Natl Acad Sci U S A. 2003; 100(22):12741-6. PMC: 240688. DOI: 10.1073/pnas.1735463100. View