Developmental Neurotoxicity of Methamidophos in the Embryo-Larval Stages of Zebrafish

Overview

Journal Int J Environ Res Public Health

Publisher MDPI

Specialties Environmental Health
Public Health

Date 2016 Dec 31

PMID 28036051

Citations 7

Authors

Xiaowei He

Jiawei Gao

Tianyu Dong

Minjian Chen

Kun Zhou

Chunxin Chang

Jia Luo

Chao Wang

Shoulin Wang

Daozhen Chen

Zuomin Zhou

Ying Tian

Yankai Xia

Xinru Wang

Affiliations

Soon will be listed here.

Abstract

Methamidophos is a representative organophosphate insecticide. The knowledge of its developmental neurotoxicity is limited, especially for zebrafish in the early stages of their life. Four hour post-fertilization (hpf) zebrafish embryos were exposed to several environmentally relevant concentrations of methamidophos (0, 25, and 500 μg/L) for up to 72 hpf. Locomotor behavior was then studied in the zebrafish larvae at this timepoint. Acridine orange (AO) staining was carried out in the zebrafish larvae, and the mRNA levels of genes associated with neural development ( and ) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The number of escape responders for mechanical stimulation was significantly decreased in exposed groups. AO staining showed noticeable signs of apoptosis mainly in the brain. In addition, the mRNA levels of and were both significantly down-regulated in exposed groups. Our study provides the first evidence that methamidophos exposure can cause developmental neurotoxicity in the early stages of zebrafish life, which may be caused by the effect of methamidophos on neurodevelopmental genes and the activation of cell apoptosis in the brain.

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