Anti-Neuroinflammatory Ent-Kaurane Diterpenoids from Pteris Multifida Roots

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2016 Dec 31

PMID 28035965

Citations 5

Authors

Jung Wha Kim

Ji Yeon Seo

Won Keun Oh

Sang Hyun Sung

Affiliations

Soon will be listed here.

Abstract

Activated microglia are known to be a major source of cellular neuroinflammation which causes various neurodegenerative diseases, including Alzheimer's disease. In our continuing efforts to search for new bioactive phytochemicals against neuroinflammatory diseases, the 80% methanolic extract of (Pteridaceae) roots was found to exhibit significant NO inhibitory activity in lipopolysaccharide (LPS)-activated BV-2 microglia cells. Three new -kaurane diterpenoids, pterokaurane M₁ 2--β-d-glucopyranoside (), 2β,16α-dihydroxy--kaurane 2,16-di--β-d-glucopyranoside (), and 2β,16α,17-trihydroxy--kaurane 2--β-d-glucopyranoside (), were isolated along with nine other known compounds from roots. The chemical structures of the new compounds were determined by 1D- and 2D-NMR, HR-ESI-MS, and CD spectroscopic data analysis. Among the isolates, compounds and significantly inhibited NO production in LPS-stimulated BV-2 cells reducing the expression of the cyclooxygenase-2 (COX-2) protein and the level of pro-inflammatory mediators such as prostaglandin E₂ (PGE₂), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6. These results suggest that -kaurane diterpenes from could be potential lead compounds that act as anti-neuroinflammatory agents.

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