SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 During Diapause Formation in Artemia

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2016 Dec 30

PMID 28031330

Citations 16

Authors

Li Dai

Sen Ye

Hua-Wei Li

Dian-Fu Chen

Hong-Liang Wang

Sheng-Nan Jia

Cheng Lin

Jin-Shu Yang

Fan Yang

Hiromichi Nagasawa

Wei-Jun Yang

Affiliations

Soon will be listed here.

Abstract

As a prominent characteristic of cell life, the regulation of cell quiescence is important for proper development, regeneration, and stress resistance and may play a role in certain degenerative diseases. However, the mechanism underlying quiescence remains largely unknown. Encysted embryos of are useful for studying the regulation of this state because they remain quiescent for prolonged periods during diapause, a state of obligate dormancy. In the present study, SET domain-containing protein 4, a histone lysine methyltransferase from , was identified, characterized, and named Ar-SETD4. We found that Ar-SETD4 was expressed abundantly in diapause embryos, in which cells were in a quiescent state. Meanwhile, trimethylated histone H4K20 (H4K20me3) was enriched in diapause embryos. The knockdown of Ar-SETD4 reduced the level of H4K20me3 significantly and prevented the formation of diapause embryos in which neither the cell cycle nor embryogenesis ceased. The catalytic activity of Ar-SETD4 on H4K20me3 was confirmed by an histone methyltransferase (HMT) assay and overexpression in cell lines. This study provides insights into the function of SETD4 and the mechanism of cell quiescence regulation.

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