Combining a Ru(II) "Building Block" and Rapid Screening Approach to Identify DNA Structure-Selective "Light Switch" Compounds

Overview

Journal ACS Comb Sci

Publisher American Chemical Society

Specialty Chemistry

Date 2016 Dec 29

PMID 28029775

Citations 2

Authors

Erin Wachter

Diego Moya

Edith C Glazer

Affiliations

Soon will be listed here.

Abstract

A chemically reactive Ru(II) "building block", able to undergo condensation reactions with substituted diamines, was utilized to create a small library of luminescent "light switch" dipyrido-[3,2-a:2',3'-c] phenazine (dppz) complexes. The impact of substituent identity, position, and the number of substituents on the light switch effect was investigated. An unbiased, parallel screening approach was used to evaluate the selectivity of the compounds for a variety of different biomolecules, including protein, nucleosides, single stranded DNA, duplex DNA, triplex DNA, and G-quadruplex DNA. Combining these two approaches allowed for the identification of hit molecules that showed different selectivities for biologically relevant DNA structures, particularly triplex and quadruplex DNA.

Citing Articles

Three's a crowd - stabilisation, structure, and applications of DNA triplexes.

Dalla Pozza M, Abdullrahman A, Cardin C, Gasser G, Hall J Chem Sci. 2022; 13(35):10193-10215.

PMID: 36277639 PMC: 9473520. DOI: 10.1039/d2sc01793h.

Ruthenium(II) Polypyridyl Complexes and Their Use as Probes and Photoreactive Agents for G-quadruplexes Labelling.

Jiang J, Teunens T, Tisaun J, Denuit L, Moucheron C Molecules. 2022; 27(5).

PMID: 35268640 PMC: 8912042. DOI: 10.3390/molecules27051541.

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