Mitoxantrone and Analogues Bind and Stabilize I-Motif Forming DNA Sequences

Overview

Journal Sci Rep

Specialty Science

Date 2016 Dec 23

PMID 28004744

Citations 21

Authors

Elise P Wright

Henry A Day

Ali M Ibrahim

Jeethendra Kumar

Leo J E Boswell

Camille Huguin

Clare E M Stevenson

Klaus Pors

Zoe A E Waller

Affiliations

Soon will be listed here.

Abstract

There are hundreds of ligands which can interact with G-quadruplex DNA, yet very few which target i-motif. To appreciate an understanding between the dynamics between these structures and how they can be affected by intervention with small molecule ligands, more i-motif binding compounds are required. Herein we describe how the drug mitoxantrone can bind, induce folding of and stabilise i-motif forming DNA sequences, even at physiological pH. Additionally, mitoxantrone was found to bind i-motif forming sequences preferentially over double helical DNA. We also describe the stabilisation properties of analogues of mitoxantrone. This offers a new family of ligands with potential for use in experiments into the structure and function of i-motif forming DNA sequences.

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