A New Formulation of Bacillus Thuringiensis: UV Protection and Sustained Release Mosquito Larvae Studies

Overview

Journal Sci Rep

Specialty Science

Date 2016 Dec 23

PMID 28004743

Citations 7

Authors

Lingling Zhang

Xiaojuan Zhang

Yi Zhang

Songqin Wu

Ivan Gelbic

Lei Xu

Xiong Guan

Affiliations

Soon will be listed here.

Abstract

Persistence of Bacillus thuringiensis is an important factor in determining the success of this product as a pest control agent. In this report we present the development of a highly active mosquitocidal formulation with high resistance to UV. LLP29-M19 strain of Bt, selected by repeated exposure to UV was found to be highly resistant to UV. The product was optimized and the methods used were statistically analyzed. Using single-factor experiments it was determined that the optimal concentration of sodium alginate, CaCl and hollow glass beads in the formulation were 1.0%, 2.0% and 3.5%, respectively. Plackett-Burman design was used to screen the interaction of the three factors, CaCl, sodium alginate and hollow glass beads in the sustained-release formulation. The best combined concentration and mutual effects of the three factors were optimized by response surface methodology. The results showed that the most favorable composition was sodium alginate 0.78%, CaCl 4.52%, hollow glass bead 3.12%, bacterial powder 3.0%, melanin 0.015%, sodium benzoate 0.2%, and mouse feed 0.5%, resulting in the immobilization time of 4.5 h, at which time the corrected sustained-release virulence rose 2391.67 fold, which was 6.07-fold higher than the basic formulation and deviated only 5.0% from the value predicted by RSM.

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References

Brar S, Verma M, Tyagi R, Valero J, Surampalli R . Screening of different adjuvants for wastewater/wastewater sludge-based Bacillus thuringiensis formulations. J Econ Entomol. 2006; 99(4):1065-79. DOI: 10.1603/0022-0493-99.4.1065. View

Liu S, Qiao L, He H, Zhang Q, Chen X, Zhou W . Optimization of fermentation conditions and rheological properties of exopolysaccharide produced by deep-sea bacterium Zunongwangia profunda SM-A87. PLoS One. 2011; 6(11):e26825. PMC: 3214017. DOI: 10.1371/journal.pone.0026825. View

Fan Y, Pereira R, Kilic E, Casella G, Keyhani N . Pyrokinin β-neuropeptide affects necrophoretic behavior in fire ants (S. invicta), and expression of β-NP in a mycoinsecticide increases its virulence. PLoS One. 2012; 7(1):e26924. PMC: 3251551. DOI: 10.1371/journal.pone.0026924. View

Cavados C, Fonseca R, Chaves J, Rabinovitch L . Identification of entomopathogenic Bacillus isolated from Simulium (Diptera, Simuliidae) larvae and adults. Mem Inst Oswaldo Cruz. 2001; 96(7):1017-21. DOI: 10.1590/s0074-02762001000700023. View

Chan L, Lee H, Heng P . Production of alginate microspheres by internal gelation using an emulsification method. Int J Pharm. 2002; 242(1-2):259-62. DOI: 10.1016/s0378-5173(02)00170-9. View

Myasnik M, Manasherob R, Ben-Dov E, Zaritsky A, Margalith Y, Barak Z . Comparative sensitivity to UV-B radiation of two Bacillus thuringiensis subspecies and other Bacillus sp. Curr Microbiol. 2001; 43(2):140-3. DOI: 10.1007/s002840010276. View

Siegwart M, Graillot B, Blachere Lopez C, Besse S, Bardin M, Nicot P . Resistance to bio-insecticides or how to enhance their sustainability: a review. Front Plant Sci. 2015; 6:381. PMC: 4472983. DOI: 10.3389/fpls.2015.00381. View

Prabakaran G, Hoti S . Immobilization in alginate as a technique for the preservation of Bacillus thuringiensis var. israelensis for long-term preservation. J Microbiol Methods. 2007; 72(1):91-4. DOI: 10.1016/j.mimet.2007.11.001. View

Kohl J, Gerlagh M, De Haas B, Krijger M . Biological Control of Botrytis cinerea in Cyclamen with Ulocladium atrum and Gliocladium roseum Under Commercial Growing Conditions. Phytopathology. 2008; 88(6):568-75. DOI: 10.1094/PHYTO.1998.88.6.568. View

10.

Wang P, Zhang C, Guo M, Guo S, Zhu Y, Zheng J . Complete genome sequence of Bacillus thuringiensis YBT-1518, a typical strain with high toxicity to nematodes. J Biotechnol. 2013; 171:1-2. DOI: 10.1016/j.jbiotec.2013.11.023. View

11.

Sansinenea E, Salazar F, Ramirez M, Ortiz A . An Ultra-Violet Tolerant Wild-Type Strain of Melanin-Producing Bacillus thuringiensis. Jundishapur J Microbiol. 2015; 8(7):e20910. PMC: 4584136. DOI: 10.5812/jjm.20910v2. View

12.

Chen Y, Deng Y, Wang J, Cai J, Ren G . Characterization of melanin produced by a wild-type strain of Bacillus thuringiensis. J Gen Appl Microbiol. 2005; 50(4):183-8. DOI: 10.2323/jgam.50.183. View

13.

Pusztai M, Fast P, Gringorten L, Kaplan H, Lessard T, Carey P . The mechanism of sunlight-mediated inactivation of Bacillus thuringiensis crystals. Biochem J. 1991; 273(Pt 1):43-7. PMC: 1149877. DOI: 10.1042/bj2730043. View

14.

Zhang J, Yan J, Zheng D, Sun Y, Yuan Z . Expression of mel gene improves the UV resistance of Bacillus thuringiensis. J Appl Microbiol. 2008; 105(1):151-7. DOI: 10.1111/j.1365-2672.2008.03729.x. View

15.

Ifoulis A, Savopoulou-Soultani M . Biological control of Lobesia botrana (Lepidoptera: Tortricidae) larvae by using different formulations of Bacillus thuringiensis in 11 vine cultivars under field conditions. J Econ Entomol. 2004; 97(2):340-3. DOI: 10.1093/jee/97.2.340. View

16.

Wu S, Lan Y, Huang D, Peng Y, Huang Z, Xu L . Use of spent mushroom substrate for production of Bacillus thuringiensis by solid-state fermentation. J Econ Entomol. 2014; 107(1):137-43. DOI: 10.1603/ec13276. View

17.

Annadurai G, Ling L, Lee J . Statistical optimization of medium components and growth conditions by response surface methodology to enhance phenol degradation by Pseudomonas putida. J Hazard Mater. 2007; 151(1):171-8. DOI: 10.1016/j.jhazmat.2007.05.061. View

18.

Zhang L, Huang E, Lin J, Gelbic I, Zhang Q, Guan Y . A novel mosquitocidal Bacillus thuringiensis strain LLP29 isolated from the phylloplane of Magnolia denudata. Microbiol Res. 2009; 165(2):133-41. DOI: 10.1016/j.micres.2009.03.002. View

19.

Koch S, Schwinger C, Kressler J, Heinzen C, Rainov N . Alginate encapsulation of genetically engineered mammalian cells: comparison of production devices, methods and microcapsule characteristics. J Microencapsul. 2003; 20(3):303-16. DOI: 10.1080/0265204021000058438. View

20.

Wu S, Lan Y, Wu Z, Peng Y, Chen S, Huang Z . Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar. Bioresour Technol. 2013; 148:596-600. DOI: 10.1016/j.biortech.2013.08.122. View