Effects of Retinoic Acid Receptor-γ on the Induced Innate Immunity Response in Human Corneal Epithelial Cells

Overview

Journal Int J Ophthalmol

Specialty Ophthalmology

Date 2016 Dec 23

PMID 28003968

Citations 2

Authors

Xiao-Chen Wang

Gui-Qiu Zhao

Jing Lin

Cui Li

Nan Jiang

Jie Zhang

Affiliations

Soon will be listed here.

Abstract

Aim: To explore the effects of retinoic acid receptor-γ (RARγ) on innate immune responses against () in cultured human corneal epithelial cells (HCECs).

Methods: The HCECs were stimulated with hyphae for 0, 2, 4, 8, 12 and 16h. RARγ mRNA and protein levels were tested by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Then HCECs were pretreated with or without BMS961 (RARγ agonist, 1 µg/mL). The mRNA and protein expression of Dectin-1 and the downstream cytokines (TNF-α and IL-6) were determined by qRT-PCR, Western blot and enzyme-linked immunosorbent assay (ELISA).

Results: The expression of RARγ was upregulated after stimulation with . RARγ mRNA began to rise at 4h and peaked at 8h (<0.001). The protein of RARγ reached to the peak at 16h (<0.001). Pretreated with BMS961 before hyphae stimulation, expression of Dectin-1, TNF-α and IL-6 decreased dramatically at mRNA and protein levels.

Conclusion: HCECs can express RARγ and hyphae infection can increase RARγ expression. BMS961 can inhibit the expression of Dectin-1 and pro-inflammatory cytokines, and play an anti-inflammatory role in innate immune responses against .

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