Temperature Dependence of Calcium-induced Fusion of Sonicated Phosphatidylserine Vesicles

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 Sep 1

PMID 279918

Citations 6

Authors

S T Sun

E P Day

J T Ho

Affiliations

Soon will be listed here.

Abstract

We have measured the temperature dependence calcium-induced fusion of sonicated phosphatidylserine vesicles. The vesicles were incubated in the presence of calcium at a specified temperature until the resulting aggregation or fusion process had gone to completion. EDTA was then added and the resulting final size of the vesicle population was measured by using dynamic light scattering. This final size was plotted against incubation temperature to show the temperature dependence of calcium-induced fusion. This curve has a peak near 11 degrees C which may be associated with the phase transition of the sonicated phosphatidylserine vesicles in the presence of calcium prior to the aggregation or fusion process.

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References

Mombers C, Van Dijck P, Van Deenen L, de Gier J, Verkleij A . The interaction of spectrin - actin and synthetic phospholipids. Biochim Biophys Acta. 1977; 470(2):152-60. DOI: 10.1016/0005-2736(77)90096-7. View

Newton C, Pangborn W, Nir S, Papahadjopoulos D . Specificity of Ca2+ and Mg2+ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure. Biochim Biophys Acta. 1978; 506(2):281-7. DOI: 10.1016/0005-2736(78)90398-x. View

Van Dijck P, de Kruijff B, Aarts P, Verkleij A, de Gier J . Phase transitions in phospholipid model membranes of different curvature. Biochim Biophys Acta. 1978; 506(2):183-91. DOI: 10.1016/0005-2736(78)90389-9. View

Papahadjopoulos D, Hui S, Vail W, Poste G . Studies on membrane fusion. I. Interactions of pure phospholipid membranes and the effect of myristic acid, lysolecithin, proteins and dimethylsulfoxide. Biochim Biophys Acta. 1976; 448(2):254-64. View

Hauser H, Finer E, Darke A . Crystalline anhydrous Ca-phosphatidylserine bilayers. Biochem Biophys Res Commun. 1976; 76(2):267-74. DOI: 10.1016/0006-291x(77)90721-5. View

Day E, Ho J, Kunze Jr R, Sun S . Dynamic light scattering study of calcium-induced fusion in phospholipid vesicles. Biochim Biophys Acta. 1977; 470(3):503-8. DOI: 10.1016/0005-2736(77)90142-0. View

KANTOR H, Mabrey S, Prestegard J, STURTEVANT J . A calorimetric examination of stable and fusing lipid bilayer vesicles. Biochim Biophys Acta. 1977; 466(3):402-10. DOI: 10.1016/0005-2736(77)90333-9. View

Verkleij A, de Kruyff B, VERVERGAERT P, Tocanne J, Van Deenen L . The influence of pH, Ca2+ and protein on the thermotropic behaviour of the negatively charged phospholipid, phosphatidylglycerol. Biochim Biophys Acta. 1974; 339(3):432-7. DOI: 10.1016/0005-2736(74)90171-0. View

Sheetz M, Chan S . Effect of sonication on the structure of lecithin bilayers. Biochemistry. 1972; 11(24):4573-81. DOI: 10.1021/bi00774a024. View

10.

Prestegard J, Fellmeth B . Fusion of dimyristoyllecithin vesicles as studied by proton magnetic resonance spectroscopy. Biochemistry. 1974; 13(6):1122-6. DOI: 10.1021/bi00703a011. View

11.

Poste G, Allison A . Membrane fusion. Biochim Biophys Acta. 1973; 300(4):421-65. DOI: 10.1016/0304-4157(73)90015-4. View

12.

Tocanne J, VERVERGAERT P, Verkleij A, Van Deenen L . A monolayer and freeze-etching study of charged phospholipids. I. Effects of ions and pH on the ionic properties of phosphatidylglycerol and lysylphosphatidylglycerol. Chem Phys Lipids. 1974; 12(3):201-19. DOI: 10.1016/0009-3084(74)90075-9. View

13.

Papahadjopoulos D, Miller N . Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals. Biochim Biophys Acta. 1967; 135(4):624-38. DOI: 10.1016/0005-2736(67)90094-6. View

14.

KANTOR H, Prestegard J . Fusion of fatty acid containing lecithin vesicles. Biochemistry. 1975; 14(8):1790-5. DOI: 10.1021/bi00679a035. View

15.

VERVERGAERT P, de Kruyff B, Verkleij A, Tocanne J, Van Deened L . Calorimetric and freeze-etch study of the influence of Mg2+ on the thermotropic behaviour of phosphatidylglycerol. Chem Phys Lipids. 1975; 14(1):97-101. DOI: 10.1016/0009-3084(75)90021-3. View

16.

Trauble H, Eibl H . Electrostatic effects on lipid phase transitions: membrane structure and ionic environment. Proc Natl Acad Sci U S A. 1974; 71(1):214-9. PMC: 387968. DOI: 10.1073/pnas.71.1.214. View

17.

Jacobson K, Papahadjopoulos D . Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations. Biochemistry. 1975; 14(1):152-61. DOI: 10.1021/bi00672a026. View

18.

Papahadjopoulos D, Vail W, Jacobson K, Poste G . Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles. Biochim Biophys Acta. 1975; 394(3):483-91. DOI: 10.1016/0005-2736(75)90299-0. View

19.

van der Bosch J, McConnell M . Fusion of dipalmitoylphosphatidylcholine vesicle membranes induced by concanavalin A. Proc Natl Acad Sci U S A. 1975; 72(11):4409-13. PMC: 388731. DOI: 10.1073/pnas.72.11.4409. View

20.

Suurkuusk J, Lentz B, Barenholz Y, Biltonen R, Thompson T . A calorimetric and fluorescent probe study of the gel-liquid crystalline phase transition in small, single-lamellar dipalmitoylphosphatidylcholine vesicles. Biochemistry. 1976; 15(7):1393-401. DOI: 10.1021/bi00652a007. View