New Cross-Talk Layer Between Ultraconserved Non-Coding RNAs, MicroRNAs and Polycomb Protein YY1 in Bladder Cancer

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2016 Dec 17

PMID 27983635

Citations 16

Authors

Sara Terreri

Montano Durso

Vincenza Colonna

Alessandra Romanelli

Daniela Terracciano

Matteo Ferro

Sisto Perdona

Luigi Castaldo

Ferdinando Febbraio

Filomena de Nigris

Amelia Cimmino

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are highly conserved elements in mammals, and exert key regulatory functions. Growing evidence shows that miRNAs can interact with another class of non-coding RNAs, so-called transcribed ultraconserved regions (T-UCRs), which take part in transcriptional, post-transcriptional and epigenetic regulation processes. We report here the interaction of miRNAs and T-UCRs as a network modulating the availability of these non-coding RNAs in bladder cancer cells. In our cell system, antagomiR-596 increased the expression of T-UCR 201+. Moreover, T-UCR 8+ silencing increased miR-596 expression, which in turn reduced total T-UCR 283+, showing that the perturbation of one element in this network changes the expression of other interactors. In addition, we identify the polycomb protein Yin Yang 1 (YY1) as mediator of binding between miR-596 and T-UCR 8+. These new findings describe for the first time a network between T-UCRs, miRNAs and YY1 protein, highlighting the existence of an additional layer of gene expression regulation.

Citing Articles

New Challenges in Bladder Cancer Diagnosis: How Biosensing Tools Can Lead to Population Screening Opportunities.

Tortora F, Guastaferro A, Barbato S, Febbraio F, Cimmino A Sensors (Basel). 2025; 24(24.

PMID: 39771612 PMC: 11679013. DOI: 10.3390/s24247873.

Emerging RNA-Based Therapeutic and Diagnostic Options: Recent Advances and Future Challenges in Genitourinary Cancers.

Tortora F, La Civita E, Trivedi P, Febbraio F, Terracciano D, Cimmino A Int J Mol Sci. 2023; 24(5).

PMID: 36902032 PMC: 10003365. DOI: 10.3390/ijms24054601.

Transcribed Ultraconserved Regions: New regulators in cancer signaling and potential biomarkers.

de Oliveira J Genet Mol Biol. 2023; 46(1 Suppl 2):e20220125.

PMID: 36622962 PMC: 9829027. DOI: 10.1590/1678-4685-GMB-2022-0125.

Role of transcribed ultraconserved regions in gastric cancer and therapeutic perspectives.

Gao S, Zhang Z, Wang X, Ma Y, Yin G, Guo X World J Gastroenterol. 2022; 28(25):2900-2909.

PMID: 35978878 PMC: 9280734. DOI: 10.3748/wjg.v28.i25.2900.

The transcribed ultraconserved region uc.160+ enhances processing and A-to-I editing of the miR-376 cluster: hypermethylation improves glioma prognosis.

Soler M, Davalos V, Sanchez-Castillo A, Mora-Martinez C, Setien F, Siqueira E Mol Oncol. 2021; 16(3):648-664.

PMID: 34665919 PMC: 8807354. DOI: 10.1002/1878-0261.13121.

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