Alpha-DNA X: Alpha and Beta Tetrathymidilates Covalently Linked to Oxazolopyridocarbazolium (OPC): Comparative Stabilization of Oligo Beta-[dT]:oligo Beta-[dA] and Oligo Alpha-[dT]:oligo Beta-[dA] Duplexes by the Intercalating Agent

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Oct 11

PMID 2798125

Citations 2

Authors

D Bazile

C Gautier

B Rayner

J L Imbach

C Paoletti

J Paoletti

Affiliations

Soon will be listed here.

Abstract

The influence of the intercalating oxazolopyridocarbazolium (HOPC) on the stabilization of modified oligonucleotides: alpha-T4c5OPC or beta-T4c5OPC associated to beta-oligo (dA) was studied. It appears that the situation is different from what has been observed for the interaction of these modified oligonucleotides with poly (rA). The higher free energy of formation of the alpha-T4c5OPC :beta-oligo(dA), when compared to beta-T4c5OPC, is essentially due to the overall stability added to this system by the intercalator. This enhanced stability comes from a higher number of binding sites of HOPC for the alpha:beta duplex together with a lower van't Hoff energy of formation of the alpha:beta duplex.

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