Use of Small-angle X-ray Scattering to Resolve Intracellular Structure Changes of Cells Induced by Antibiotic Treatment

Overview

Journal J Appl Crystallogr

Date 2016 Dec 17

PMID 27980516

Citations 10

Authors

A R von Gundlach

V M Garamus

T M Willey

J Ilavsky

K Hilpert

A Rosenhahn

Affiliations

Soon will be listed here.

Abstract

The application of small-angle X-ray scattering (SAXS) to whole cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of was developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins. was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.

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