Portulacerebroside A Inhibits Adhesion, Migration, and Invasion of Human Leukemia HL60 Cells and U937 Cells Through the Regulation of P38/JNK Signaling Pathway

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2016 Dec 14

PMID 27956839

Citations 4

Authors

Qidong Ye

Xuelian Liao

Pan Fu

Jiaying Dou

Kai Chen

Hui Jiang

Affiliations

Soon will be listed here.

Abstract

Acute myeloid leukemia (AML) is a highly malignant hematopoietic tumor. This study aimed to explore the effect of portulacerebroside A (PCA) on the adhesion, migration, and invasion in human leukemia HL60 cells and U937 cells and clarify the possible mechanisms involved, which could provide potential strategies for the treatment of AML. By methyl thiazolyl tetrazolium analysis, it was found that PCA (1-10 μM) suppressed the cell viability in a time- and dose-dependent manner. A total of 1, 2, and 5 μM of PCA dramatically inhibited the adhesion, migration, and invasion of HL60 cells and U937 cells in a dose-dependent manner. Phosphorylation level of JNK and P38 protein level was measured by Western blot. After the real-time quantification polymerase chain reaction and Western blot detection of the total RNA and protein, messenger RNA, and protein expression levels of Ras homologous C (RhoC), metastasis-associated gene 1 (MTA1) and matrix metalloproteinase-2/9 (MMP-2/9) were decreased significantly in a dose-dependent manner. The phosphorylation level of c-Jun N-terminal kinase (JNK) and P38 mitogen-activated protein kinase (P38) was decreased dramatically in HL60 cells and U937 cells after PCA treatment. In conclusion, PCA significantly inhibits the adhesion, migration, and invasion of HL60 cells and U937 cells by suppressing the p38/JNK pathway and regulating the expressions of related genes.

Citing Articles

Disturbing cytoskeleton by engineered nanomaterials for enhanced cancer therapeutics.

Xu X, Xu S, Wan J, Wang D, Pang X, Gao Y Bioact Mater. 2023; 29:50-71.

PMID: 37621771 PMC: 10444958. DOI: 10.1016/j.bioactmat.2023.06.016.

Upregulation of hsa-miR-625-5p Inhibits Invasion of Acute Myeloid Leukemia Cancer Cells through ILK/AKT Pathway.

Samieyan Dehkordi S, Mousavi S, Ebrahimi M, Alizadeh S, Hedayati Asl A, Mohammad M Cell J. 2022; 24(2):76-84.

PMID: 35279963 PMC: 8918266. DOI: 10.22074/cellj.2022.7658.

Tanshinone IIA induces apoptosis and autophagy in acute monocytic leukemia via downregulation of PI3K/Akt pathway.

Zhang Y, Geng Y, He J, Wu D, Zhang T, Xue L Am J Transl Res. 2019; 11(5):2995-3006.

PMID: 31217869 PMC: 6556636.

Overexpression of PRDM5 promotes acute myeloid leukemia cell proliferation and migration by activating the JNK pathway.

Zhou P, Chen X, Li M, Sun X, Tan J, Wang X Cancer Med. 2019; 8(8):3905-3917.

PMID: 31119897 PMC: 6639193. DOI: 10.1002/cam4.2261.

References

Vardiman J, Lee Harris N, Brunning R . The World Health Organization (WHO) classification of the myeloid neoplasms. Blood. 2002; 100(7):2292-302. DOI: 10.1182/blood-2002-04-1199. View

Wu R, Wang Z, Liu M, Chen D, Yue X . beta2-integrins mediate a novel form of chemoresistance in cycloheximide-induced U937 apoptosis. Cell Mol Life Sci. 2004; 61(16):2071-82. PMC: 11138690. DOI: 10.1007/s00018-004-4165-1. View

Wu Z, Lien J, Huang Y, Liao C, Lin J, Fan M . Casticin Inhibits A375.S2 Human Melanoma Cell Migration/Invasion through Downregulating NF-κB and Matrix Metalloproteinase-2 and -1. Molecules. 2016; 21(3):384. PMC: 6274196. DOI: 10.3390/molecules21030384. View

Kolch W, Calder M, Gilbert D . When kinases meet mathematics: the systems biology of MAPK signalling. FEBS Lett. 2005; 579(8):1891-5. DOI: 10.1016/j.febslet.2005.02.002. View

Ikoma T, Takahashi T, Nagano S, Li Y, Ohno Y, Ando K . A definitive role of RhoC in metastasis of orthotopic lung cancer in mice. Clin Cancer Res. 2004; 10(3):1192-200. DOI: 10.1158/1078-0432.ccr-03-0275. View

Lei X, Li J, Liu B, Zhang N, Liu H . Separation and Identification of Four New Compounds with Antibacterial Activity from Portulaca oleracea L. Molecules. 2015; 20(9):16375-87. PMC: 6332283. DOI: 10.3390/molecules200916375. View

Jalmi S, Sinha A . ROS mediated MAPK signaling in abiotic and biotic stress- striking similarities and differences. Front Plant Sci. 2015; 6:769. PMC: 4585162. DOI: 10.3389/fpls.2015.00769. View

Sasaki H, Moriyama S, Nakashima Y, Kobayashi Y, Yukiue H, Kaji M . Expression of the MTA1 mRNA in advanced lung cancer. Lung Cancer. 2002; 35(2):149-54. DOI: 10.1016/s0169-5002(01)00329-4. View

Hofer M, Kuefer R, Varambally S, Li H, Ma J, Shapiro G . The role of metastasis-associated protein 1 in prostate cancer progression. Cancer Res. 2004; 64(3):825-9. DOI: 10.1158/0008-5472.can-03-2755. View

10.

Roberts P, Der C . Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 2007; 26(22):3291-310. DOI: 10.1038/sj.onc.1210422. View

11.

Ye Q, Zhang N, Chen K, Zhu J, Jiang H . Effects of portulacerebroside a on apoptosis of human leukemia HL60 cells and p38/JNK signaling pathway. Int J Clin Exp Pathol. 2016; 8(11):13968-77. PMC: 4713494. View

12.

Lan C, Chen M, Tai K, Yu D, Yang Y, Jan P . Functional microarray analysis of differentially expressed genes in granulosa cells from women with polycystic ovary syndrome related to MAPK/ERK signaling. Sci Rep. 2015; 5:14994. PMC: 4602237. DOI: 10.1038/srep14994. View

13.

Marcucci G, Caligiuri M, Bloomfield C . Molecular and clinical advances in core binding factor primary acute myeloid leukemia: a paradigm for translational research in malignant hematology. Cancer Invest. 2000; 18(8):768-80. DOI: 10.3109/07357900009012209. View

14.

El-Sayed M . Effects of Portulaca oleracea L. seeds in treatment of type-2 diabetes mellitus patients as adjunctive and alternative therapy. J Ethnopharmacol. 2011; 137(1):643-51. DOI: 10.1016/j.jep.2011.06.020. View

15.

Munshi H, Wu Y, Mukhopadhyay S, Ottaviano A, Sassano A, Koblinski J . Differential regulation of membrane type 1-matrix metalloproteinase activity by ERK 1/2- and p38 MAPK-modulated tissue inhibitor of metalloproteinases 2 expression controls transforming growth factor-beta1-induced pericellular collagenolysis. J Biol Chem. 2004; 279(37):39042-50. DOI: 10.1074/jbc.M404958200. View

16.

Zhao W, Lu M, Zhang Q . Chloride intracellular channel 1 regulates migration and invasion in gastric cancer by triggering the ROS-mediated p38 MAPK signaling pathway. Mol Med Rep. 2015; 12(6):8041-7. PMC: 4758331. DOI: 10.3892/mmr.2015.4459. View

17.

Handra-Luca A, Mauguen A, Menard P, Fouret P . Coordinated expression of activated mitogen-activated protein kinases in salivary gland adenoid cystic carcinoma. Hum Pathol. 2008; 39(11):1590-6. DOI: 10.1016/j.humpath.2008.04.008. View

18.

Ji Q, Zheng G, Xia W, Chen J, Meng X, Zhang H . Inhibition of invasion and metastasis of human liver cancer HCCLM3 cells by portulacerebroside A. Pharm Biol. 2014; 53(5):773-80. DOI: 10.3109/13880209.2014.941505. View

19.

Yan J, Sun L, Zhou Z, Chen Y, Zhang W, Dai H . Homoisoflavonoids from the medicinal plant Portulaca oleracea. Phytochemistry. 2012; 80:37-41. DOI: 10.1016/j.phytochem.2012.05.014. View

20.

Cano E, Mahadevan L . Parallel signal processing among mammalian MAPKs. Trends Biochem Sci. 1995; 20(3):117-22. DOI: 10.1016/s0968-0004(00)88978-1. View