The Impact of Environmental Parameters on Microcystin Production in Dialysis Bag Experiments

Overview

Journal Sci Rep

Specialty Science

Date 2016 Dec 10

PMID 27934931

Citations 4

Authors

Liqiang Xie

Richard R Rediske

Nadia D Gillett

James P OKeefe

Brian Scull

Qingju Xue

Affiliations

Soon will be listed here.

Abstract

It is important to understand what environmental parameters may regulate microcystin (MC) production and congener type. To determine if environmental conditions in two hydraulically connected lakes can influence MC production and congener ratios, we incubated dialysis bags containing phytoplankton from mesotrophic/eutrophic Muskegon Lake into hypereutrophic Bear Lake (Michigan, USA) and vice versa. Strong cyanobacteria growth was observed in all dialysis bags with Bear Lake phytoplankton in July and August. Phytoplankton communities were dominated by Aphanizomenon aphanizomenoides, Microcystis wesenbergii, Limnothrix redekei. MC concentrations were correlated with M. wesenbergii and A. aphanizomenoides biovolume. MC concentrations in bags incubated in the Muskegon Lake with Bear Lake water were significantly higher than the other bags. The higher light intensity and total nitrogen concentration may have caused the increase of MC production. The MC-LR/MC-RR ratios varied with sample origin but not with lake of incubation, indicating that physical environmental factors (water temperature and turbidity) were not the reasons for different toxin production ratios. Differences in total phosphorus concentrations might be one reason for the dissimilarity of the MC-LR/MC-RR ratio between the two lakes. The higher light intensity and NO-N concentration in Muskegon Lake are two factors contributing to an increase of MC production.

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References

Oh H, Lee S, Jang M, Yoon B . Microcystin production by Microcystis aeruginosa in a phosphorus-limited chemostat. Appl Environ Microbiol. 2000; 66(1):176-9. PMC: 91802. DOI: 10.1128/AEM.66.1.176-179.2000. View

Long B, Jones G, Orr P . Cellular microcystin content in N-limited Microcystis aeruginosa can be predicted from growth rate. Appl Environ Microbiol. 2001; 67(1):278-83. PMC: 92564. DOI: 10.1128/AEM.67.1.278-283.2001. View

Kurmayer R, Dittmann E, Fastner J, Chorus I . Diversity of microcystin genes within a population of the toxic cyanobacterium Microcystis spp. in Lake Wannsee (Berlin, Germany). Microb Ecol. 2002; 43(1):107-18. DOI: 10.1007/s00248-001-0039-3. View

Barco M, Rivera J, Caixach J . Analysis of cyanobacterial hepatotoxins in water samples by microbore reversed-phase liquid chromatography-electrospray ionisation mass spectrometry. J Chromatogr A. 2002; 959(1-2):103-11. DOI: 10.1016/s0021-9673(02)00405-3. View

Wiedner C, Visser P, Fastner J, Metcalf J, Codd G, Mur L . Effects of light on the microcystin content of Microcystis strain PCC 7806. Appl Environ Microbiol. 2003; 69(3):1475-81. PMC: 150065. DOI: 10.1128/AEM.69.3.1475-1481.2003. View

Paerl H, Fulton 3rd R, Moisander P, Dyble J . Harmful freshwater algal blooms, with an emphasis on cyanobacteria. ScientificWorldJournal. 2003; 1:76-113. PMC: 6083932. DOI: 10.1100/tsw.2001.16. View

de Figueiredo D, Azeiteiro U, Esteves S, Goncalves F, Pereira M . Microcystin-producing blooms--a serious global public health issue. Ecotoxicol Environ Saf. 2004; 59(2):151-63. DOI: 10.1016/j.ecoenv.2004.04.006. View

Via-Ordorika L, Fastner J, Kurmayer R, Hisbergues M, Dittmann E, Komarek J . Distribution of microcystin-producing and non-microcystin-producing Microcystis sp. in European freshwater bodies: detection of microcystins and microcystin genes in individual colonies. Syst Appl Microbiol. 2004; 27(5):592-602. DOI: 10.1078/0723202041748163. View

Tonk L, Visser P, Christiansen G, Dittmann E, Snelder E, Wiedner C . The microcystin composition of the cyanobacterium Planktothrix agardhii changes toward a more toxic variant with increasing light intensity. Appl Environ Microbiol. 2005; 71(9):5177-81. PMC: 1214630. DOI: 10.1128/AEM.71.9.5177-5181.2005. View

10.

Wilson A, Sarnelle O, Neilan B, Salmon T, Gehringer M, Hay M . Genetic variation of the bloom-forming Cyanobacterium Microcystis aeruginosa within and among lakes: implications for harmful algal blooms. Appl Environ Microbiol. 2005; 71(10):6126-33. PMC: 1265933. DOI: 10.1128/AEM.71.10.6126-6133.2005. View

11.

Jezbera J, Hornak K, Simek K . Prey selectivity of bacterivorous protists in different size fractions of reservoir water amended with nutrients. Environ Microbiol. 2006; 8(8):1330-9. DOI: 10.1111/j.1462-2920.2006.01026.x. View

12.

Xie L, Yokoyama A, Nakamura K, Park H . Accumulation of microcystins in various organs of the freshwater snail Sinotaia histrica and three fishes in a temperate lake, the eutrophic Lake Suwa, Japan. Toxicon. 2006; 49(5):646-52. DOI: 10.1016/j.toxicon.2006.11.004. View

13.

Xu Y, Wu Z, Yu B, Peng X, Yu G, Wei Z . Non-microcystin producing Microcystis wesenbergii (Komárek) Komárek (Cyanobacteria) representing a main waterbloom-forming species in Chinese waters. Environ Pollut. 2008; 156(1):162-7. DOI: 10.1016/j.envpol.2007.12.027. View

14.

Dyble J, Fahnenstiel G, Litaker R, Millie D, Tester P . Microcystin concentrations and genetic diversity of Microcystis in the lower Great Lakes. Environ Toxicol. 2008; 23(4):507-16. DOI: 10.1002/tox.20370. View

15.

Tonk L, Van de Waal D, Slot P, Huisman J, Matthijs H, Visser P . Amino acid availability determines the ratio of microcystin variants in the cyanobacterium Planktothrix agardhii. FEMS Microbiol Ecol. 2008; 65(3):383-90. DOI: 10.1111/j.1574-6941.2008.00541.x. View

16.

Backer L, Carmichael W, Kirkpatrick B, Williams C, Irvin M, Zhou Y . Recreational exposure to low concentrations of microcystins during an algal bloom in a small lake. Mar Drugs. 2008; 6(2):389-406. PMC: 2525495. DOI: 10.3390/md20080018. View

17.

Zhang D, Xie P, Liu Y, Qiu T . Transfer, distribution and bioaccumulation of microcystins in the aquatic food web in Lake Taihu, China, with potential risks to human health. Sci Total Environ. 2009; 407(7):2191-9. DOI: 10.1016/j.scitotenv.2008.12.039. View

18.

Van de Waal D, Verspagen J, Lurling M, van Donk E, Visser P, Huisman J . The ecological stoichiometry of toxins produced by harmful cyanobacteria: an experimental test of the carbon-nutrient balance hypothesis. Ecol Lett. 2009; 12(12):1326-35. DOI: 10.1111/j.1461-0248.2009.01383.x. View

19.

Miller M, Kudela R, Mekebri A, Crane D, Oates S, Tinker M . Evidence for a novel marine harmful algal bloom: cyanotoxin (microcystin) transfer from land to sea otters. PLoS One. 2010; 5(9). PMC: 2936937. DOI: 10.1371/journal.pone.0012576. View

20.

Wang Q, Niu Y, Xie P, Chen J, Ma Z, Tao M . Factors affecting temporal and spatial variations of microcystins in Gonghu Bay of Lake Taihu, with potential risk of microcystin contamination to human health. ScientificWorldJournal. 2010; 10:1795-809. PMC: 5763806. DOI: 10.1100/tsw.2010.172. View