Effects of Trimethylamine -oxide and Urea on DNA Duplex and G-quadruplex

Overview

Journal Sci Technol Adv Mater

Specialty Biomedical Engineering

Date 2016 Dec 10

PMID 27933115

Citations 5

Authors

Yu-Mi Ueda

Yu-Ki Zouzumi

Atsushi Maruyama

Shu-Ichi Nakano

Naoki Sugimoto

Daisuke Miyoshi

Affiliations

Soon will be listed here.

Abstract

We systematically investigated effects of molecular crowding with trimethylamine -oxide (TMAO) as a zwitterionic and protective osmolyte and urea as a nonionic denaturing osmolyte on conformation and thermodynamics of the canonical DNA duplex and the non-canonical DNA G-quadruplex. It was found that TMAO and urea stabilized and destabilized, respectively, the G-quadruplex. On the other hand, these osmolytes generally destabilize the duplex; however, it was observed that osmolytes having the trimethylamine group stabilized the duplex at the lower concentrations because of a direct binding to a groove of the duplex. These results are useful not only to predict DNA structures and their thermodynamics under physiological environments in living cells, but also design of polymers and materials to regulate structure and stability of DNA sequences.

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