Screening of Long Non-coding RNA and TUG1 Inhibits Proliferation with TGF-β Induction in Patients with COPD

Overview

Journal Int J Chron Obstruct Pulmon Dis

Publisher Dove Medical Press

Specialty Pulmonary Medicine

Date 2016 Dec 10

PMID 27932875

Citations 39

Authors

Wenxiang Tang

Zhenyu Shen

Jiang Guo

Shenghua Sun

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate differentially expressed long noncoding RNAs (lncRNAs) and the potential role of lncRNA TUG1 in patients with chronic obstructive pulmonary disease (COPD).

Methods: Total RNA was extracted from both COPD and non-COPD lung tissues, and microarray analysis was performed with 25,628 lncRNA probes and 20,106 mRNA probes. In addition, five up-regulated and five down-regulated lncRNAs were selected for identification using quantitative real-time polymerase chain reaction. COPD cell model was established by transforming growth factor β (TGF-β) treatment. Cell Counting Kit-8 assay was used to detect BEAS-2B and HFL1 cell proliferation after TUG-siRNA transfection with TGF-β treatment. In addition, the expression levels of α-SMA and fibronectin proteins were determined using Western blot in BEAS-2B and HFL1 cells after TUG-siRNA transfection with TGF-β treatment.

Results: There were 8,376 (32.7%) differentially expressed lncRNAs and 5,094 (25.3%) differentially expressed mRNAs in COPD lung tissues compared with non-COPD lung tissues. Five of the analyzed lncRNAs (BC038205, BC130595, TUG1, MEG3, and LOC646329) were markedly increased, while five lncRNAs (LOC729178, PLAC2, LOC339529, LINC00229, and SNHG5) were significantly decreased in COPD lung tissues compared with non-COPD lung tissues (n=20) (<0.001). Knockdown of lncRNA TUG1 promotes BEAS-2B and HFL1 cell proliferation after TGF-β treatment through inhibiting the expression levels of α-SMA and fibronectin.

Conclusion: Abundant, differentially expressed lncRNAs and mRNAs were identified by microarray analysis and these might play a partial or key role in the diagnosis of patients with COPD. LncRNA TUG1 may become a very important class of biomarker and may act as a potential diagnostic and therapeutic target for patients with COPD.

Citing Articles

Analysis of the basement membrane-related genes ITGA7 and its regulatory role in periodontitis via machine learning: a retrospective study.

Ye H, Gao X, Ma Y, He S, Zhou Z BMC Oral Health. 2024; 24(1):1548.

PMID: 39719569 PMC: 11669225. DOI: 10.1186/s12903-024-05201-w.

Treatment of chronic obstructive pulmonary disease by traditional Chinese medicine Morin monomer regulated by autophagy.

Liu Z, Zeng Y, Li R, Yan Y, Yi S, Zhang K J Thorac Dis. 2024; 16(9):6052-6063.

PMID: 39444855 PMC: 11494543. DOI: 10.21037/jtd-23-1836.

Decoding LncRNA in COPD: Unveiling Prognostic and Diagnostic Power and Their Driving Role in Lung Cancer Progression.

Sweef O, Mahfouz R, Tascioglu T, Albowaidey A, Abdelmonem M, Asfar M Int J Mol Sci. 2024; 25(16).

PMID: 39201688 PMC: 11354875. DOI: 10.3390/ijms25169001.

Long non-coding RNAs in biomarking COVID-19: a machine learning-based approach.

Heydari R, Tavassolifar M, Fayazzadeh S, Sadatpour O, Meyfour A Virol J. 2024; 21(1):134.

PMID: 38849961 PMC: 11161961. DOI: 10.1186/s12985-024-02408-9.

Cigarette Smoke Extract-Treated Mouse Airway Epithelial Cells-Derived Exosomal LncRNA MEG3 Promotes M1 Macrophage Polarization and Pyroptosis in Chronic Obstructive Pulmonary Disease by Upregulating TREM-1 via mA Methylation.

Wang L, Yu Q, Xiao J, Chen Q, Fang M, Zhao H Immune Netw. 2024; 24(2):e3.

PMID: 38725674 PMC: 11076299. DOI: 10.4110/in.2024.24.e3.

References

Taft R, Pang K, Mercer T, Dinger M, Mattick J . Non-coding RNAs: regulators of disease. J Pathol. 2009; 220(2):126-39. DOI: 10.1002/path.2638. View

Simone R, Russo M, Catalano A, Monego G, Froehlich K, Boehm V . Lycopene inhibits NF-kB-mediated IL-8 expression and changes redox and PPARγ signalling in cigarette smoke-stimulated macrophages. PLoS One. 2011; 6(5):e19652. PMC: 3098254. DOI: 10.1371/journal.pone.0019652. View

Cao W, Wu H, He B, Zhang Y, Zhang Z . Analysis of long non-coding RNA expression profiles in gastric cancer. World J Gastroenterol. 2013; 19(23):3658-64. PMC: 3691033. DOI: 10.3748/wjg.v19.i23.3658. View

Pauwels R, Buist A, Calverley P, Jenkins C, Hurd S . Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI/WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary. Am J Respir Crit Care Med. 2001; 163(5):1256-76. DOI: 10.1164/ajrccm.163.5.2101039. View

Mattick J . The genetic signatures of noncoding RNAs. PLoS Genet. 2009; 5(4):e1000459. PMC: 2667263. DOI: 10.1371/journal.pgen.1000459. View

Wang P, Ren Z, Sun P . Overexpression of the long non-coding RNA MEG3 impairs in vitro glioma cell proliferation. J Cell Biochem. 2012; 113(6):1868-74. DOI: 10.1002/jcb.24055. View

Kim A, Zhao H, Ifrim I, Dean A . Beta-globin intergenic transcription and histone acetylation dependent on an enhancer. Mol Cell Biol. 2007; 27(8):2980-6. PMC: 1899946. DOI: 10.1128/MCB.02337-06. View

. American Thoracic Society. Idiopathic pulmonary fibrosis: diagnosis and treatment. International consensus statement. American Thoracic Society (ATS), and the European Respiratory Society (ERS). Am J Respir Crit Care Med. 2000; 161(2 Pt 1):646-64. DOI: 10.1164/ajrccm.161.2.ats3-00. View

Wang K, Chang H . Molecular mechanisms of long noncoding RNAs. Mol Cell. 2011; 43(6):904-14. PMC: 3199020. DOI: 10.1016/j.molcel.2011.08.018. View

10.

Liu S, Nowakowski T, Pollen A, Lui J, Horlbeck M, Attenello F . Single-cell analysis of long non-coding RNAs in the developing human neocortex. Genome Biol. 2016; 17:67. PMC: 4831157. DOI: 10.1186/s13059-016-0932-1. View

11.

Cabili M, Trapnell C, Goff L, Koziol M, Tazon-Vega B, Regev A . Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses. Genes Dev. 2011; 25(18):1915-27. PMC: 3185964. DOI: 10.1101/gad.17446611. View

12.

Lorenowicz M, Fernandez-Borja M, Hordijk P . cAMP signaling in leukocyte transendothelial migration. Arterioscler Thromb Vasc Biol. 2007; 27(5):1014-22. DOI: 10.1161/ATVBAHA.106.132282. View

13.

Derrien T, Johnson R, Bussotti G, Tanzer A, Djebali S, Tilgner H . The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression. Genome Res. 2012; 22(9):1775-89. PMC: 3431493. DOI: 10.1101/gr.132159.111. View

14.

Cao G, Zhang J, Wang M, Song X, Liu W, Mao C . Differential expression of long non-coding RNAs in bleomycin-induced lung fibrosis. Int J Mol Med. 2013; 32(2):355-64. DOI: 10.3892/ijmm.2013.1404. View

15.

Guttman M, Amit I, Garber M, French C, Lin M, Feldser D . Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature. 2009; 458(7235):223-7. PMC: 2754849. DOI: 10.1038/nature07672. View

16.

Congrains A, Kamide K, Oguro R, Yasuda O, Miyata K, Yamamoto E . Genetic variants at the 9p21 locus contribute to atherosclerosis through modulation of ANRIL and CDKN2A/B. Atherosclerosis. 2011; 220(2):449-55. DOI: 10.1016/j.atherosclerosis.2011.11.017. View

17.

Tufarelli C, Stanley J, Garrick D, Sharpe J, Ayyub H, Wood W . Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease. Nat Genet. 2003; 34(2):157-65. DOI: 10.1038/ng1157. View

18.

Xu Y, Wang J, Qiu M, Xu L, Li M, Jiang F . Upregulation of the long noncoding RNA TUG1 promotes proliferation and migration of esophageal squamous cell carcinoma. Tumour Biol. 2014; 36(3):1643-51. DOI: 10.1007/s13277-014-2763-6. View

19.

Poller W, Kuhl U, Tschoepe C, Pauschinger M, Fechner H, Schultheiss H . Genome-environment interactions in the molecular pathogenesis of dilated cardiomyopathy. J Mol Med (Berl). 2005; 83(8):579-86. DOI: 10.1007/s00109-005-0664-2. View

20.

Lahzami S, Aubert J . Lung transplantation for COPD - evidence-based?. Swiss Med Wkly. 2009; 139(1-2):4-8. DOI: 10.4414/smw.2009.12377. View