Compressive Loading of the Murine Tibia Reveals Site-specific Micro-scale Differences in Adaptation and Maturation Rates of Bone

Overview

Journal Osteoporos Int

Specialties Endocrinology
Orthopedics

Date 2016 Dec 7

PMID 27921145

Citations 8

Authors

I Bergstrom

J G Kerns

A E Tornqvist

C Perdikouri

N Mathavan

A Koskela

H B Henriksson

J Tuukkanen

G Andersson

H Isaksson

A E Goodship

S H Windahl

Affiliations

Soon will be listed here.

Abstract

Introduction: Osteoporosis is a degenerative disease resulting in reduced bone mineral density, structure, and strength. The overall aim was to explore the hypothesis that changes in loading environment result in site-specific adaptations at molecular/micro- and macro-scale in mouse bone.

Methods: Right tibiae of adult mice were subjected to well-defined cyclic axial loading for 2 weeks; left tibiae were used as physiologically loaded controls. The bones were analyzed with μCT (structure), reference point indentation (material properties), Raman spectroscopy (chemical), and small-angle X-ray scattering (mineral crystallization and structure).

Results: The cranial and caudal sites of tibiae are structurally and biochemically different within control bones. In response to loading, cranial and caudal sites increase in cortical thickness with reduced mineralization (-14 and -3%, p < 0.01, respectively) and crystallinity (-1.4 and -0.3%, p < 0.05, respectively). Along the length of the loaded bones, collagen content becomes more heterogeneous on the caudal site and the mineral/collagen increases distally at both sites.

Conclusion: Bone structure and composition are heterogeneous, finely tuned, adaptive, and site-specifically responsive at the micro-scale to maintain optimal function. Manipulation of this heterogeneity may affect bone strength, relative to specific applied loads.

Citing Articles

Strain Distribution Evaluation of Rat Tibia under Axial Compressive Load by Combining Strain Gauge Measurement and Finite Element Analysis.

Gao J, Liu B, Zhang M, Gong H, Gao B Appl Bionics Biomech. 2019; 2019:1736763.

PMID: 31871486 PMC: 6913262. DOI: 10.1155/2019/1736763.

αSMA Osteoprogenitor Cells Contribute to the Increase in Osteoblast Numbers in Response to Mechanical Loading.

Matthews B, Wee N, Widjaja V, Price J, Kalajzic I, Windahl S Calcif Tissue Int. 2019; 106(2):208-217.

PMID: 31673746 PMC: 6995756. DOI: 10.1007/s00223-019-00624-y.

Murine Axial Compression Tibial Loading Model to Study Bone Mechanobiology: Implementing the Model and Reporting Results.

Main R, Shefelbine S, Meakin L, Silva M, van der Meulen M, Willie B J Orthop Res. 2019; 38(2):233-252.

PMID: 31508836 PMC: 9344861. DOI: 10.1002/jor.24466.

Experimental mechanical strain measurement of tissues.

Huang L, Korhonen R, Turunen M, Finnila M PeerJ. 2019; 7:e6545.

PMID: 30867989 PMC: 6409087. DOI: 10.7717/peerj.6545.

Treadmill running and targeted tibial loading differentially improve bone mass in mice.

Berman A, Hinton M, Wallace J Bone Rep. 2019; 10:100195.

PMID: 30701187 PMC: 6348199. DOI: 10.1016/j.bonr.2019.100195.

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