Blockade of IL-23 Ameliorates Allergic Lung Inflammation Via Decreasing the Infiltration of Tc17 Cells

Overview

Journal Arch Med Sci

Specialty General Medicine

Date 2016 Dec 2

PMID 27904530

Citations 6

Authors

Sheng Cheng

Huilong Chen

Aili Wang

Hansvin Bunjhoo

Yong Cao

Jungang Xie

Yongjian Xu

Weining Xiong

Affiliations

Soon will be listed here.

Abstract

Introduction: Tc17 cells are interleukin (IL)-17-producing CD8 T cells and have been found to participate in the development of allergic asthma. Interleukin-23 is a cytokine that may be involved in modulating the IL-17 response via Th17 cells. This study aimed to investigate whether IL-23 also has immunomodulatory effects on Tc17 cells.

Material And Methods: An allergic asthmatic mouse model was induced by sensitizing and challenging with ovalbumin (OVA). Anti-IL-23 antibody was administered intratracheally before challenge to the OVA-induced asthmatic mouse model. Airway hyperresponsiveness, lung inflammation, Tc17 cell percentages and IL-17 level in the lung tissue homogenate were measured.

Results: Anti-IL-23 treatment reduced airway hyperresponsiveness (Rn 2.471 ±0.5077 vs. 4.051 ±0.2334, < 0.05), inflammatory cell infiltration in bronchoalveolar lavage fluid (eosinophils 140.0 ±9.869 vs. 222.4 ±31.55, < 0.05, neutrophils 75.93 ±6.745 vs. 127.4 ±19.73, < 0.05), airway inflammation and mucus secretion. Treatment with anti-IL-23 antibody also markedly reduced IL-17 level (398.1 ±28.74 vs. 590.6 ±36.13, < 0.01) and percentage of Th17 and Tc17 cells in lung tissue homogenate (4.200 ±0.1581 vs. 9.314 ±1.027, < 0.01 and 2.852 ±0.2566 vs. 5.588 ±0.3631, < 0.01, Th17 and Tc17 cells respectively).

Conclusions: Our data suggest that the IL-23/Tc17 cell axis may be involved in the pathogenesis of asthma as the complement of IL-23/Th17 cells.

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References

Wilson R, Whitehead G, Nakano H, Free M, Kolls J, Cook D . Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness. Am J Respir Crit Care Med. 2009; 180(8):720-30. PMC: 2778149. DOI: 10.1164/rccm.200904-0573OC. View

Al-Ramli W, Prefontaine D, Chouiali F, Martin J, Olivenstein R, Lemiere C . T(H)17-associated cytokines (IL-17A and IL-17F) in severe asthma. J Allergy Clin Immunol. 2009; 123(5):1185-7. DOI: 10.1016/j.jaci.2009.02.024. View

Agrawal A, Rengarajan S, Adler K, Ram A, Ghosh B, Fahim M . Inhibition of mucin secretion with MARCKS-related peptide improves airway obstruction in a mouse model of asthma. J Appl Physiol (1985). 2006; 102(1):399-405. DOI: 10.1152/japplphysiol.00630.2006. View

McGeachy M, Bak-Jensen K, Chen Y, Tato C, Blumenschein W, Mcclanahan T . TGF-beta and IL-6 drive the production of IL-17 and IL-10 by T cells and restrain T(H)-17 cell-mediated pathology. Nat Immunol. 2007; 8(12):1390-7. DOI: 10.1038/ni1539. View

He D, Wu L, Kim H, Li H, Elmets C, Xu H . CD8+ IL-17-producing T cells are important in effector functions for the elicitation of contact hypersensitivity responses. J Immunol. 2006; 177(10):6852-8. PMC: 3179908. DOI: 10.4049/jimmunol.177.10.6852. View

You Q, Zhang D, Niu C, Zhu Z, Wang N, Yue Y . Expression of IL-17A and IL-17F in lipopolysaccharide-induced acute lung injury and the counteraction of anisodamine or methylprednisolone. Cytokine. 2014; 66(1):78-86. DOI: 10.1016/j.cyto.2013.12.019. View

Schnyder-Candrian S, Togbe D, Couillin I, Mercier I, Brombacher F, Quesniaux V . Interleukin-17 is a negative regulator of established allergic asthma. J Exp Med. 2006; 203(12):2715-25. PMC: 2118159. DOI: 10.1084/jem.20061401. View

Barnes P . Immunology of asthma and chronic obstructive pulmonary disease. Nat Rev Immunol. 2008; 8(3):183-92. DOI: 10.1038/nri2254. View

He R, Oyoshi M, Jin H, Geha R . Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge. Proc Natl Acad Sci U S A. 2007; 104(40):15817-22. PMC: 2000444. DOI: 10.1073/pnas.0706942104. View

10.

Li K, Wang Z, Cao Y, Bunjhoo H, Zhu J, Chen Y . The study of the ratio and distribution of Th17 cells and Tc17 cells in asthmatic patients and the mouse model. Asian Pac J Allergy Immunol. 2013; 31(2):125-31. DOI: 10.12932/AP0268.31.2.2013. View

11.

Nakagome K, Imamura M, Okada H, Kawahata K, Inoue T, Hashimoto K . Dopamine D1-like receptor antagonist attenuates Th17-mediated immune response and ovalbumin antigen-induced neutrophilic airway inflammation. J Immunol. 2011; 186(10):5975-82. DOI: 10.4049/jimmunol.1001274. View

12.

Huber M, Heink S, Pagenstecher A, Reinhard K, Ritter J, Visekruna A . IL-17A secretion by CD8+ T cells supports Th17-mediated autoimmune encephalomyelitis. J Clin Invest. 2012; 123(1):247-60. PMC: 3533283. DOI: 10.1172/JCI63681. View

13.

Penido C, Costa M, Souza M, Costa K, Candea A, Benjamim C . Involvement of CC chemokines in gammadelta T lymphocyte trafficking during allergic inflammation: the role of CCL2/CCR2 pathway. Int Immunol. 2007; 20(1):129-39. DOI: 10.1093/intimm/dxm128. View

14.

Henriques A, Ines L, Couto M, Pedreiro S, Santos C, Magalhaes M . Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus. Cell Immunol. 2010; 264(1):97-103. DOI: 10.1016/j.cellimm.2010.05.004. View

15.

Bullens D, Truyen E, Coteur L, Dilissen E, Hellings P, Dupont L . IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx?. Respir Res. 2006; 7:135. PMC: 1636037. DOI: 10.1186/1465-9921-7-135. View

16.

Sarra M, Pallone F, Macdonald T, Monteleone G . IL-23/IL-17 axis in IBD. Inflamm Bowel Dis. 2010; 16(10):1808-13. DOI: 10.1002/ibd.21248. View

17.

Galli S, Tsai M, Piliponsky A . The development of allergic inflammation. Nature. 2008; 454(7203):445-54. PMC: 3573758. DOI: 10.1038/nature07204. View

18.

Lewkowich I, Lajoie S, Clark J, Herman N, Sproles A, Wills-Karp M . Allergen uptake, activation, and IL-23 production by pulmonary myeloid DCs drives airway hyperresponsiveness in asthma-susceptible mice. PLoS One. 2008; 3(12):e3879. PMC: 2586658. DOI: 10.1371/journal.pone.0003879. View

19.

Chalubinski M, Wojdan K, Dorantowicz R, Jackowska P, Gorzelak P, Broncel M . Comprehensive insight into immune regulatory mechanisms and vascular wall determinants of atherogenesis - emerging perspectives of immunomodulation. Arch Med Sci. 2013; 9(1):159-65. PMC: 3598149. DOI: 10.5114/aoms.2013.33355. View

20.

Htun Oo Y, Banz V, Kavanagh D, Liaskou E, Withers D, Humphreys E . CXCR3-dependent recruitment and CCR6-mediated positioning of Th-17 cells in the inflamed liver. J Hepatol. 2012; 57(5):1044-51. PMC: 3994510. DOI: 10.1016/j.jhep.2012.07.008. View