Determination of Scattering Properties and Damage Thresholds in Tissue Using Ultrafast Laser Ablation

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2016 Dec 1

PMID 27901549

Citations 5

Authors

Chris Martin

Adela Ben-Yakar

Affiliations

Soon will be listed here.

Abstract

Ultrafast laser surgery of tissue requires precise knowledge of the tissue’s optical properties to control the extent of subsurface ablation. Here, we present a method to determine the scattering lengths, ?s, and fluence thresholds, Fth, in multilayered and turbid tissue by finding the input energies required to initiate ablation at various depths in each tissue layer. We validated the method using tissue-mimicking phantoms and applied it to porcine vocal folds, which consist of an epithelial (ep) layer and a superficial lamina propia (SLP) layer. Across five vocal fold samples, we found ?s,ep=51.0±3.9???m, Fth,ep=1.78±0.08??J/cm2, ?s,SLP=26.5±1.6???m, and Fth,SLP=1.14±0.12??J/cm2. Our method can enable personalized determination of tissue optical properties in a clinical setting, leading to less patient-to-patient variability and more favorable outcomes in operations, such as femto-LASIK surgery.

Citing Articles

Ultrafast laser surgery probe for sub-surface ablation to enable biomaterial injection in vocal folds.

Andrus L, Jeon H, Pawlowski M, Debord B, Gerome F, Benabid F Sci Rep. 2022; 12(1):20554.

PMID: 36446830 PMC: 9708667. DOI: 10.1038/s41598-022-24446-5.

In vivo hamster cheek pouch subepithelial ablation, biomaterial injection, and localization: pilot study.

Gabay I, Subramanian K, Andrus L, DuPlissis A, Yildirim M, Ben-Yakar A J Biomed Opt. 2022; 27(8).

PMID: 36008882 PMC: 9407625. DOI: 10.1117/1.JBO.27.8.080501.

Quantitative third-harmonic generation imaging of mouse visual cortex areas reveals correlations between functional maps and structural substrates.

Yildirim M, Hu M, Le N, Sugihara H, So P, Sur M Biomed Opt Express. 2020; 11(10):5650-5673.

PMID: 33149977 PMC: 7587247. DOI: 10.1364/BOE.396962.

Scattering properties and femtosecond laser ablation thresholds of human and canine vocal folds at 776-nm wavelength.

Andrus L, Mau T, Ben-Yakar A J Biomed Opt. 2019; 24(8):1-7.

PMID: 31468749 PMC: 6983523. DOI: 10.1117/1.JBO.24.8.085005.

Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy.

Yildirim M, Sugihara H, So P, Sur M Nat Commun. 2019; 10(1):177.

PMID: 30635577 PMC: 6329792. DOI: 10.1038/s41467-018-08179-6.

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