The Effect of Isoflurane on F-FDG Uptake in the Rat Brain: a Fully Conscious Dynamic PET Study Using Motion Compensation

Overview

Journal EJNMMI Res

Date 2016 Nov 27

PMID 27888500

Citations 16

Authors

Matthew G Spangler-Bickell

Bart de Laat

Roger Fulton

Guy Bormans

Johan Nuyts

Affiliations

Soon will be listed here.

Abstract

Background: In preclinical positron emission tomography (PET) studies an anaesthetic is used to ensure that the animal does not move during the scan. However, anaesthesia may have confounding effects on the drug or tracer kinetics under study, and the nature of these effects is usually not known.

Method: We have implemented a protocol for tracking the rigid motion of the head of a fully conscious rat during a PET scan and performing a motion compensated list-mode reconstruction of the data. Using this technique we have conducted eight rat studies to investigate the effect of isoflurane on the uptake of F-FDG in the brain, by comparing conscious and unconscious scans.

Results: Our results indicate that isoflurane significantly decreases the whole brain uptake, as well as decreasing the relative regional FDG uptake in the cortex, diencephalon, and inferior colliculi, while increasing it in the vestibular nuclei. No statistically significant changes in FDG uptake were observed in the cerebellum and striata.

Conclusion: The applied event-based motion compensation technique allowed for the investigation of the effect of isoflurane on FDG uptake in the rat brain using fully awake and unrestrained rats, scanned dynamically from the moment of injection. A significant effect of the anaesthesia was observed in various regions of the brain.

Citing Articles

Positron emission tomography neuroimaging of [F]fluorodeoxyglucose uptake and related behavior in the -/- rat model of Parkinson disease.

Converse A, Krasko M, Rudisch D, Lunaris C, Nisbet A, Slesarev M Front Neurosci. 2024; 18:1451118.

PMID: 39474461 PMC: 11520326. DOI: 10.3389/fnins.2024.1451118.

Dynamic neuroreceptor positron emission tomography in non-anesthetized rats using point source based motion correction: A feasibility study with [C]ABP688.

Kroll T, Miranda A, Drechsel A, Beer S, Lang M, Drzezga A J Cereb Blood Flow Metab. 2024; 44(10):1852-1866.

PMID: 38684219 PMC: 11504418. DOI: 10.1177/0271678X241239133.

Evaluation of Brain [F]F-FDG Uptake Pattern Under Different Anaesthesia Protocols.

Kepes Z, Arato V, Csikos C, Hegedus E, Esze R, Nagy T In Vivo. 2024; 38(2):587-597.

PMID: 38418149 PMC: 10905451. DOI: 10.21873/invivo.13477.

Effects of isoflurane anaesthesia depth and duration on renal function measured with [Tc]Tc-mercaptoacetyltriglycine SPECT in mice.

Schmitz-Peiffer F, Lukas M, Mohan A, Albrecht J, Aschenbach J, Brenner W EJNMMI Res. 2024; 14(1):4.

PMID: 38180547 PMC: 10769950. DOI: 10.1186/s13550-023-01065-3.

Metabolic brain imaging with glucosamine CEST MRI: in vivo characterization and first insights.

Rivlin M, Perlman O, Navon G Sci Rep. 2023; 13(1):22030.

PMID: 38086821 PMC: 10716494. DOI: 10.1038/s41598-023-48515-5.

References

Hosoi R, Matsumura A, Mizokawa S, Tanaka M, Nakamura F, Kobayashi K . MicroPET detection of enhanced 18F-FDG utilization by PKA inhibitor in awake rat brain. Brain Res. 2005; 1039(1-2):199-202. DOI: 10.1016/j.brainres.2005.01.064. View

Fueger B, Czernin J, Hildebrandt I, Tran C, Halpern B, Stout D . Impact of animal handling on the results of 18F-FDG PET studies in mice. J Nucl Med. 2006; 47(6):999-1006. View

McLaughlin K, Gomez J, Baran S, Conrad C . The effects of chronic stress on hippocampal morphology and function: an evaluation of chronic restraint paradigms. Brain Res. 2007; 1161:56-64. PMC: 2667378. DOI: 10.1016/j.brainres.2007.05.042. View

Alstrup A, Smith D . Anaesthesia for positron emission tomography scanning of animal brains. Lab Anim. 2013; 47(1):12-8. DOI: 10.1258/la.2012.011173. View

Patel V, Lee D, Alexoff D, Dewey S, Schiffer W . Imaging dopamine release with Positron Emission Tomography (PET) and (11)C-raclopride in freely moving animals. Neuroimage. 2008; 41(3):1051-66. DOI: 10.1016/j.neuroimage.2008.02.065. View

Matsumura A, Mizokawa S, Tanaka M, Wada Y, Nozaki S, Nakamura F . Assessment of microPET performance in analyzing the rat brain under different types of anesthesia: comparison between quantitative data obtained with microPET and ex vivo autoradiography. Neuroimage. 2003; 20(4):2040-50. DOI: 10.1016/j.neuroimage.2003.08.020. View

Spangler-Bickell M, Zhou L, Kyme A, de Laat B, Fulton R, Nuyts J . Optimising rigid motion compensation for small animal brain PET imaging. Phys Med Biol. 2016; 61(19):7074-7091. DOI: 10.1088/0031-9155/61/19/7074. View

Hildebrandt I, Su H, Weber W . Anesthesia and other considerations for in vivo imaging of small animals. ILAR J. 2008; 49(1):17-26. DOI: 10.1093/ilar.49.1.17. View

Deleye S, Verhaeghe J, Wyffels L, Dedeurwaerdere S, Stroobants S, Staelens S . Towards a reproducible protocol for repetitive and semi-quantitative rat brain imaging with (18) F-FDG: exemplified in a memantine pharmacological challenge. Neuroimage. 2014; 96:276-87. DOI: 10.1016/j.neuroimage.2014.04.004. View

10.

Kyme A, Zhou V, Meikle S, Baldock C, Fulton R . Optimised motion tracking for positron emission tomography studies of brain function in awake rats. PLoS One. 2011; 6(7):e21727. PMC: 3128597. DOI: 10.1371/journal.pone.0021727. View

11.

Tai Y, Ruangma A, Rowland D, Siegel S, Newport D, Chow P . Performance evaluation of the microPET focus: a third-generation microPET scanner dedicated to animal imaging. J Nucl Med. 2005; 46(3):455-63. View

12.

Diltoer M, Camu F . Glucose homeostasis and insulin secretion during isoflurane anesthesia in humans. Anesthesiology. 1988; 68(6):880-6. DOI: 10.1097/00000542-198806000-00008. View

13.

Hudson H, Larkin R . Accelerated image reconstruction using ordered subsets of projection data. IEEE Trans Med Imaging. 1994; 13(4):601-9. DOI: 10.1109/42.363108. View

14.

Schulz D, Southekal S, Junnarkar S, Pratte J, Purschke M, Stoll S . Simultaneous assessment of rodent behavior and neurochemistry using a miniature positron emission tomograph. Nat Methods. 2011; 8(4):347-52. DOI: 10.1038/nmeth.1582. View

15.

Loepke A, McCann J, Kurth C, McAuliffe J . The physiologic effects of isoflurane anesthesia in neonatal mice. Anesth Analg. 2005; 102(1):75-80. DOI: 10.1213/01.ANE.0000181102.92729.B8. View

16.

Wong K, Sha W, Zhang X, Huang S . Effects of administration route, dietary condition, and blood glucose level on kinetics and uptake of 18F-FDG in mice. J Nucl Med. 2011; 52(5):800-7. PMC: 3086987. DOI: 10.2967/jnumed.110.085092. View

17.

Casteels C, Vunckx K, Aelvoet S, Baekelandt V, Bormans G, Van Laere K . Construction and evaluation of quantitative small-animal PET probabilistic atlases for [¹⁸F]FDG and [¹⁸F]FECT functional mapping of the mouse brain. PLoS One. 2013; 8(6):e65286. PMC: 3676471. DOI: 10.1371/journal.pone.0065286. View

18.

Gupta R, Schafer C, Ramaroson Y, Sciullo M, Horn C . Role of the abdominal vagus and hindbrain in inhalational anesthesia-induced vomiting. Auton Neurosci. 2016; 202:114-121. PMC: 5203944. DOI: 10.1016/j.autneu.2016.06.007. View

19.

Kyme A, Se S, Meikle S, Angelis G, Ryder W, Popovic K . Markerless motion tracking of awake animals in positron emission tomography. IEEE Trans Med Imaging. 2014; 33(11):2180-90. DOI: 10.1109/TMI.2014.2332821. View

20.

Shepp L, Vardi Y . Maximum likelihood reconstruction for emission tomography. IEEE Trans Med Imaging. 1982; 1(2):113-22. DOI: 10.1109/TMI.1982.4307558. View