Rapid in Situ Toxicity Testing with Luminescent Bacteria Photorhabdus Luminescens and Vibrio Fischeri Adapted to a Small Portable Luminometer

Overview

Journal Environ Sci Pollut Res Int

Publisher Springer

Specialties Environmental Health
Toxicology

Date 2016 Nov 27

PMID 27888485

Citations 1

Authors

Petr Masner

Barbora Javurkova

Ludek Blaha

Affiliations

Soon will be listed here.

Abstract

The present study demonstrates development of a rapid testing protocol based on a small portable luminometer using flash kinetic assessment of bacterial bioluminescence. The laboratory comparisons based on six model organic toxicants and two metals showed significant correlations between responses of freshwater bacteria Photorhabdus luminescens and standard marine bacterial species Vibrio fisheri. While P. luminescens was less sensitive in standard arrangements, the responses of both organisms were comparable in the newly introduced portable luminometer setup. The applicability and reproducibility of the portable luminometer protocol was further demonstrated in the assessment of 43 European wastewater effluents that were simultaneously tested for toxicity and analysed for 150 organic and 20 inorganic contaminants grouped into 13 major chemical classes. Clear association between the toxic responses in both compared bacterial species and the elevated levels of inorganic compounds (toxic metals), chlorophenols and benzotriazole anticorrosives was observed. The new protocol with a portable luminometer provides a fast (30 s) response and may be used as a tool for rapid in situ toxicity evaluation of freshwater environmental samples such as effluents.

Citing Articles

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Rashdan H, Shehadi I, Abdelrahman M, Hemdan B Molecules. 2021; 26(16).

PMID: 34443405 PMC: 8399954. DOI: 10.3390/molecules26164817.

References

Kurvet I, Ivask A, Bondarenko O, Sihtmae M, Kahru A . LuxCDABE--transformed constitutively bioluminescent Escherichia coli for toxicity screening: comparison with naturally luminous Vibrio fischeri. Sensors (Basel). 2011; 11(8):7865-78. PMC: 3231736. DOI: 10.3390/s110807865. View

Campisi T, Abbondanzi F, Casado-Martinez C, DelValls T, Guerra R, Iacondini A . Effect of sediment turbidity and color on light output measurement for Microtox Basic Solid-Phase Test. Chemosphere. 2005; 60(1):9-15. DOI: 10.1016/j.chemosphere.2004.12.052. View

Lappalainen J, Juvonen R, Nurmi J, Karp M . Automated color correction method for Vibrio fischeri toxicity test. Comparison of standard and kinetic assays. Chemosphere. 2001; 45(4-5):635-41. DOI: 10.1016/s0045-6535(00)00579-8. View

Farre M, Martinez E, Hernando M, Fernandez-Alba A, Fritz J, Unruh E . European ring exercise on water toxicity using different bioluminescence inhibition tests based on Vibrio fischeri, in support to the implementation of the water framework directive. Talanta. 2008; 69(2):323-33. DOI: 10.1016/j.talanta.2005.09.047. View

Rosal R, Rodea-Palomares I, Boltes K, Fernandez-Pinas F, Leganes F, Gonzalo S . Ecotoxicity assessment of lipid regulators in water and biologically treated wastewater using three aquatic organisms. Environ Sci Pollut Res Int. 2009; 17(1):135-44. DOI: 10.1007/s11356-009-0137-1. View

Thavamani P, Megharaj M, Naidu R . Metal-tolerant PAH-degrading bacteria: development of suitable test medium and effect of cadmium and its availability on PAH biodegradation. Environ Sci Pollut Res Int. 2013; 22(12):8957-68. DOI: 10.1007/s11356-013-1850-3. View

Dunlap P . Osmotic control of luminescence and growth in Photobacterium leiognathi from ponyfish light organs. Arch Microbiol. 1985; 141(1):44-50. DOI: 10.1007/BF00446738. View

Muller R, Schreiber U, Escher B, Quayle P, Bengtson Nash S, Mueller J . Rapid exposure assessment of PSII herbicides in surface water using a novel chlorophyll a fluorescence imaging assay. Sci Total Environ. 2008; 401(1-3):51-9. DOI: 10.1016/j.scitotenv.2008.02.062. View

Escher B, Allinson M, Altenburger R, Bain P, Balaguer P, Busch W . Benchmarking organic micropollutants in wastewater, recycled water and drinking water with in vitro bioassays. Environ Sci Technol. 2013; 48(3):1940-56. DOI: 10.1021/es403899t. View

10.

Suresh Kumar K, Dahms H, Lee J, Kim H, Lee W, Shin K . Algal photosynthetic responses to toxic metals and herbicides assessed by chlorophyll a fluorescence. Ecotoxicol Environ Saf. 2014; 104:51-71. DOI: 10.1016/j.ecoenv.2014.01.042. View

11.

Pandard P, Devillers J, Charissou A, Poulsen V, Jourdain M, Ferard J . Selecting a battery of bioassays for ecotoxicological characterization of wastes. Sci Total Environ. 2006; 363(1-3):114-25. DOI: 10.1016/j.scitotenv.2005.12.016. View

12.

Jarosova B, Ersekova A, Hilscherova K, Loos R, Gawlik B, Giesy J . Europe-wide survey of estrogenicity in wastewater treatment plant effluents: the need for the effect-based monitoring. Environ Sci Pollut Res Int. 2014; 21(18):10970-82. DOI: 10.1007/s11356-014-3056-8. View

13.

Loos R, Carvalho R, Antonio D, Comero S, Locoro G, Tavazzi S . EU-wide monitoring survey on emerging polar organic contaminants in wastewater treatment plant effluents. Water Res. 2013; 47(17):6475-87. DOI: 10.1016/j.watres.2013.08.024. View

14.

Pessala P, Schultz E, Nakari T, Joutti A, Herve S . Evaluation of wastewater effluents by small-scale biotests and a fractionation procedure. Ecotoxicol Environ Saf. 2004; 59(2):263-72. DOI: 10.1016/j.ecoenv.2003.10.002. View

15.

Escher B, van Daele C, Dutt M, Tang J, Altenburger R . Most oxidative stress response in water samples comes from unknown chemicals: the need for effect-based water quality trigger values. Environ Sci Technol. 2013; 47(13):7002-11. DOI: 10.1021/es304793h. View

16.

Stewart G, Williams P . lux genes and the applications of bacterial bioluminescence. J Gen Microbiol. 1992; 138(7):1289-300. DOI: 10.1099/00221287-138-7-1289. View

17.

Jennings V, Bird D . Assessing chemical toxicity with the bioluminescent photobacterium (Vibrio fischeri): a comparison of three commercial systems. Water Res. 2001; 35(14):3448-56. DOI: 10.1016/s0043-1354(01)00067-7. View

18.

Berglind R, Leffler P, Sjostrom M . Interactions between pH, potassium, calcium, bromide, and phenol and their effects on the bioluminescence of Vibrio fischeri. J Toxicol Environ Health A. 2010; 73(16):1102-12. DOI: 10.1080/15287394.2010.482918. View

19.

Dizer H, WITTEKINDT E, Fischer B, Hansen P . The cytotoxic and genotoxic potential of surface water and wastewater effluents as determined by bioluminescence, umu-assays and selected biomarkers. Chemosphere. 2002; 46(2):225-33. DOI: 10.1016/s0045-6535(01)00062-5. View

20.

Volpi Ghirardini A, Girardini M, Marchetto D, Pantani C . Microtox solid phase test: Effect of diluent used in toxicity test. Ecotoxicol Environ Saf. 2008; 72(3):851-61. DOI: 10.1016/j.ecoenv.2008.01.011. View