Aqueous Immune Mediators in Malignant Uveal Melanomas in Comparison to Benign Pigmented Intraocular Tumors

Overview

Journal Graefes Arch Clin Exp Ophthalmol

Specialty Ophthalmology

Date 2016 Nov 24

PMID 27878431

Citations 6

Authors

Yoshihiko Usui

Kinya Tsubota

Tsuyoshi Agawa

Shunichiro Ueda

Kazuhiko Umazume

Yoko Okunuki

Takeshi Kezuka

Naoyuki Yamakawa

Hiroshi Goto

Affiliations

Soon will be listed here.

Abstract

Background: To examine the usefulness of measuring immune mediators in aqueous humor samples for differentiating malignant uveal melanoma from benign pigmented intraocular tumors.

Methods: Thirteen eyes of 13 patients with uveal melanoma were studied, and 13 eyes of 13 patients with benign pigmented intraocular tumors served as controls. Undiluted samples of aqueous humor were collected, and a cytometric bead array was used to determine the aqueous humor concentrations of 35 immune mediators comprising 14 interleukins (IL), interferon-γ, interferon-γ-inducible protein-10, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1α, MIP-1β, regulated on activation normal T cell expressed and secreted, monokine induced by interferon-γ, basic fibroblast growth factor, Fas ligand, granzyme A, granzyme B, eotaxin, interferon-inducible T-cell alpha chemoattractant, fractalkine, granulocyte macrophage colony-stimulating factor, granulocyte colony-stimulating factor, vascular endothelial growth factor, angiogenin, tumor necrosis factor-α, lymphotoxin-α, and CD40L.

Results: Aqueous humor levels of angiogenin, IL-8, and MCP-1 were significantly higher in eyes with malignant melanoma than in those with benign tumors (p < 0.05).

Conclusions: Angiogenin, IL-8, and MCP-1 levels in aqueous humor may be potential markers for distinguishing malignant uveal melanoma from benign pigmented intraocular tumors, and may be a useful adjunct to histomorphology, diagnostic imaging, and other biomarkers for the diagnosis and appropriate clinical management of malignant uveal melanoma.

Citing Articles

Genetic Features of Uveal Melanoma.

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Expression of GNAQ, BAP1, SF3B1, and EIF1AX Proteins in the Aqueous Humor of Eyes Affected by Uveal Melanoma.

Midena G, Parrozzani R, Frizziero L, Esposito G, Micera A, Midena E Invest Ophthalmol Vis Sci. 2024; 65(1):15.

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Variability of Replicates of Intraocular Inflammatory Biomarkers in Ocular Fluid Samples Analyzed with Multiplex Assays.

Lamoureux D, Wong D, Felfeli T Clin Ophthalmol. 2023; 17:2653-2663.

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Genetic and Epigenetic Features of Uveal Melanoma-An Overview and Clinical Implications.

Pasalic D, Nikuseva-Martic T, Sekovanic A, Kastelan S Int J Mol Sci. 2023; 24(16).

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Investigating Vitreous Cytokines in Choroidal Melanoma.

Demirci H, Tang L, Demirci F, Ozgonul C, Weber S, Sundstrom J Cancers (Basel). 2023; 15(14).

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