Stem Cell Technology Using Bioceramics: Hard Tissue Regeneration Towards Clinical Application

Overview

Journal Sci Technol Adv Mater

Specialty Biomedical Engineering

Date 2016 Nov 24

PMID 27877325

Citations 5

Authors

Hiroe Ohnishi

Yasuaki Oda

Hajime Ohgushi

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stem cells (MSCs) are adult stem cells which show differentiation capabilities toward various cell lineages. We have already used MSCs for treatments of osteoarthritis, bone necrosis and bone tumor. For this purpose, culture expanded MSCs were combined with various ceramics and then implanted. Because of rejection response to allogeneic MSC implantation, we have utilized patients' own MSCs for the treatment. Bone marrow is a good cell source of MSCs, although the MSCs also exist in adipose tissue. When comparing osteogenic differentiation of these MSCs, bone marrow MSCs show more extensive bone forming capability than adipose MSCs. Thus, the bone marrow MSCs are useful for bone tissue regeneration. However, the MSCs show limited proliferation and differentiation capabilities that hindered clinical applications in some cases. Recent advances reveal that transduction of plural transcription factors into human adult cells results in generation of new type of stem cells called induced pluripotent stem cells (iPS cells). A drawback of the iPS cells for clinical applications is tumor formation after their implantation; therefore it is difficult to use iPS cells for the treatment. To circumvent the problem, we transduced a single factor of either or into the MSCs and found high proliferation as well as osteogenic differentiation capabilities of the MSCs. The stem cells could be combined with bioceramics for clinical applications. Here, we summarize our recent technologies using adult stem cells in viewpoints of bone tissue regeneration.

Citing Articles

A novel biologically hierarchical hydrogel with osteoblast precursor-targeting extracellular vesicles ameliorates bone loss in vivo via the sequential action of antagomiR-200b-3p and antagomiR-130b-3p.

Dai H, Yu Y, Han J, Luo J, Song C, Deng Z Cell Prolif. 2023; 56(8):e13426.

PMID: 36786008 PMC: 10392057. DOI: 10.1111/cpr.13426.

Mesenchymal Stromal/Stem Cell (MSC)-Based Vector Biomaterials for Clinical Tissue Engineering and Inflammation Research: A Narrative Mini Review.

Xue J, Liu Y J Inflamm Res. 2023; 16:257-267.

PMID: 36713049 PMC: 9875582. DOI: 10.2147/JIR.S396064.

Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration.

Ding X, Yang G, Zhang W, Li G, Lin S, Kaplan D Sci Rep. 2017; 7(1):2175.

PMID: 28526887 PMC: 5438390. DOI: 10.1038/s41598-017-02053-z.

pH-responsive polymeric micelles with core-shell-corona architectures as intracellular anti-cancer drug carriers.

Prasad Bastakoti B, Liao S, Inoue M, Yusa S, Imura M, Nakashima K Sci Technol Adv Mater. 2016; 14(4):044402.

PMID: 27877587 PMC: 5090313. DOI: 10.1088/1468-6996/14/4/044402.

Assembly of cells and vesicles for organ engineering.

Taguchi T Sci Technol Adv Mater. 2016; 12(6):064703.

PMID: 27877453 PMC: 5090668. DOI: 10.1088/1468-6996/12/6/064703.

References

Kotobuki N, Katsube Y, Katou Y, Tadokoro M, Hirose M, Ohgushi H . In vivo survival and osteogenic differentiation of allogeneic rat bone marrow mesenchymal stem cells (MSCs). Cell Transplant. 2008; 17(6):705-12. DOI: 10.3727/096368908786092793. View

Halvorsen Y, Wilkison W, Gimble J . Adipose-derived stromal cells--their utility and potential in bone formation. Int J Obes Relat Metab Disord. 2000; 24 Suppl 4:S41-4. DOI: 10.1038/sj.ijo.0801503. View

Episkopou V . SOX2 functions in adult neural stem cells. Trends Neurosci. 2005; 28(5):219-21. DOI: 10.1016/j.tins.2005.03.003. View

Rasmusson I, Ringden O, Sundberg B, Le Blanc K . Mesenchymal stem cells inhibit lymphocyte proliferation by mitogens and alloantigens by different mechanisms. Exp Cell Res. 2005; 305(1):33-41. DOI: 10.1016/j.yexcr.2004.12.013. View

Arinzeh T, Peter S, Archambault M, van den Bos C, Gordon S, Kraus K . Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect. J Bone Joint Surg Am. 2003; 85(10):1927-35. DOI: 10.2106/00004623-200310000-00010. View

Bradley A, Evans M, Kaufman M, Robertson E . Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature. 1984; 309(5965):255-6. DOI: 10.1038/309255a0. View

Guillot P, Gotherstrom C, Chan J, Kurata H, Fisk N . Human first-trimester fetal MSC express pluripotency markers and grow faster and have longer telomeres than adult MSC. Stem Cells. 2006; 25(3):646-54. DOI: 10.1634/stemcells.2006-0208. View

Erickson G, Gimble J, Franklin D, Rice H, Awad H, Guilak F . Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun. 2002; 290(2):763-9. DOI: 10.1006/bbrc.2001.6270. View

Martin G . Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A. 1981; 78(12):7634-8. PMC: 349323. DOI: 10.1073/pnas.78.12.7634. View

10.

Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K . Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007; 131(5):861-72. DOI: 10.1016/j.cell.2007.11.019. View

11.

Hayashi O, Katsube Y, Hirose M, Ohgushi H, Ito H . Comparison of osteogenic ability of rat mesenchymal stem cells from bone marrow, periosteum, and adipose tissue. Calcif Tissue Int. 2008; 82(3):238-47. DOI: 10.1007/s00223-008-9112-y. View

12.

Tadokoro M, Kanai R, Taketani T, Uchio Y, Yamaguchi S, Ohgushi H . New bone formation by allogeneic mesenchymal stem cell transplantation in a patient with perinatal hypophosphatasia. J Pediatr. 2009; 154(6):924-30. DOI: 10.1016/j.jpeds.2008.12.021. View

13.

Yu J, Vodyanik M, Smuga-Otto K, Antosiewicz-Bourget J, Frane J, Tian S . Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007; 318(5858):1917-20. DOI: 10.1126/science.1151526. View

14.

Siminovitch L, McCulloch E, Till J . THE DISTRIBUTION OF COLONY-FORMING CELLS AMONG SPLEEN COLONIES. J Cell Comp Physiol. 1963; 62:327-36. DOI: 10.1002/jcp.1030620313. View

15.

FRIEDENSTEIN A, PETRAKOVA K, Kurolesova A, Frolova G . Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968; 6(2):230-47. View

16.

Okita K, Ichisaka T, Yamanaka S . Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448(7151):313-7. DOI: 10.1038/nature05934. View

17.

Ohgushi H, Dohi Y, Katuda T, Tamai S, Tabata S, Suwa Y . In vitro bone formation by rat marrow cell culture. J Biomed Mater Res. 1996; 32(3):333-40. DOI: 10.1002/(SICI)1097-4636(199611)32:3<333::AID-JBM5>3.0.CO;2-T. View

18.

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

19.

Safford K, Hicok K, Safford S, Halvorsen Y, Wilkison W, Gimble J . Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem Biophys Res Commun. 2002; 294(2):371-9. DOI: 10.1016/S0006-291X(02)00469-2. View

20.

Ohgushi H, Kotobuki N, Funaoka H, Machida H, Hirose M, Tanaka Y . Tissue engineered ceramic artificial joint--ex vivo osteogenic differentiation of patient mesenchymal cells on total ankle joints for treatment of osteoarthritis. Biomaterials. 2005; 26(22):4654-61. DOI: 10.1016/j.biomaterials.2004.11.055. View