HMGA1P7-pseudogene Regulates H19 and Igf2 Expression by a Competitive Endogenous RNA Mechanism

Overview

Journal Sci Rep

Specialty Science

Date 2016 Nov 23

PMID 27874091

Citations 23

Authors

Marco De Martino

Floriana Forzati

Marianna Marfella

Simona Pellecchia

Claudio Arra

Luigi Terracciano

Alfredo Fusco

Francesco Esposito

Affiliations

Soon will be listed here.

Abstract

Recent studies have revealed that pseudogene transcripts can function as competing endogenous RNAs, and thereby can also contribute to cancer when dysregulated. We have recently identified two pseudogenes, HMGA1P6 and HMGA1P7 for the HMGA1 gene whose overexpression has a critical role in cancer progression. These pseudogenes work as competitive endogenous RNA decoys for HMGA1 and other cancer related genes suggesting their role in carcinogenesis. Looking for new HMGA1 pseudogene ceRNAs, we performed RNA sequencing technology on mouse embryonic fibroblasts deriving from transgenic mice overexpressing HMGA1P7. Here, we report that HMGA1P7 mRNA sustains the H19 and Igf2 overexpression by acting as miRNA decoy. Lastly, the expression of HMGA1P7 was significantly correlated with H19 and IGF2 levels in human breast cancer thereby suggesting a role for HMGA1P7 deregulation in this neoplasia.

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