Vertebrate Host Susceptibility to Heartland Virus

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2016 Nov 22

PMID 27869591

Citations 31

Authors

Angela M Bosco-Lauth

Amanda E Calvert

J Jeffrey Root

Tom Gidlewski

Brian H Bird

Richard A Bowen

Atis Muehlenbachs

Sherif R Zaki

Aaron C Brault

Affiliations

Soon will be listed here.

Abstract

Heartland virus (HRTV) is a recently described phlebovirus initially isolated in 2009 from 2 humans who had leukopenia and thrombocytopenia. Serologic assessment of domestic and wild animal populations near the residence of 1 of these persons showed high exposure rates to raccoons, white-tailed deer, and horses. To our knowledge, no laboratory-based assessments of viremic potential of animals infected with HRTV have been performed. We experimentally inoculated several vertebrates (raccoons, goats, chickens, rabbits, hamsters, C57BL/6 mice, and interferon-α/β/γ receptor-deficient [Ag129]) mice with this virus. All animals showed immune responses against HRTV after primary or secondary exposure. However, neutralizing antibody responses were limited. Only Ag129 mice showed detectable viremia and associated illness and death, which were dose dependent. Ag129 mice also showed development of mean peak viral antibody titers >8 log PFU/mL, hemorrhagic hepatic lesions, splenomegaly, and large amounts of HRTV antigen in mononuclear cells and hematopoietic cells in the spleen.

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