Rapid Detection of Viral Antibodies Based on Multifunctional Staphylococcus Aureus Nanobioprobes

Overview

Journal Enzyme Microb Technol

Publisher Elsevier

Date 2016 Nov 22

PMID 27866631

Citations 6

Authors

Jinjuan Qiao

Yunpeng Li

Cuihua Wei

Hang Yang

Junping Yu

Hongping Wei

Affiliations

Soon will be listed here.

Abstract

Biosynthesis of nanoparticles inside S. aureus cells has enhanced the sensitivity of immunoassays based on the S. aureus nanoparticles. However, the current methods are limited to antigen detection by conjugating IgG antibodies on S. aureus nanoparticles. In this study, a simple way to conjugate antigens to the S. aureus nanobioparticles was developed by utilizing a cell wall binding domain (CBD) from a bacteriophage lysin PlyV12. Based on this novel design, simple agglutination tests of the IgG antibodies of Ebola virus (EBOV) nucleoprotein (NP) and Middle East Respiratory Virus (MERS) NP in rabbit sera were successfully developed by conjugating the S. aureus nanobioparticles with two fusion proteins EBOV NP- CBD and MERS NP-CBD, respectively. The conjugation was done easily by just mixing the fusion proteins with the S. aureus nanoparticles. The detection time was within 20 min without any special equipment or expertise. As far as we know, this is the first time to realize the detection of viral antibodies based on S. aureus nanoparticles.

Citing Articles

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