Sesamin Inhibits IL-1β-stimulated Inflammatory Response in Human Osteoarthritis Chondrocytes by Activating Nrf2 Signaling Pathway

Overview

Journal Oncotarget

Specialty Oncology

Date 2016 Nov 19

PMID 27863411

Citations 32

Authors

Pengyu Kong

Guanghua Chen

Anlong Jiang

Yufu Wang

Chengchao Song

Jinpeng Zhuang

Chunyang Xi

Guangxi Wang

Ye Ji

Jinglong Yan

Affiliations

Soon will be listed here.

Abstract

Sesamin, a bioactive component extracted from sesame, has been reported to exert anti-inflammatory and anti-oxidant effects. In this study, we evaluated the anti-inflammatory effects of sesamin on IL-1β-stimulated human osteoarthritis chondrocytes and investigated the possible mechanism. Results demonstrated that sesamin treatment significantly inhibited PGE2 and NO production induced by IL-1β. Sesamin inhibited MMP1, MMP3, and MMP13 production in IL-1β-stimulated chondrocytes. Sesamin also inhibited IL-1β-induced phosphorylation of NF-κB p65 and IκBα. Meanwhile, sesamin was found to up-regulate the expression of Nrf2 and HO-1. However, Nrf2 siRNA reversed the anti-inflammatory effects of sesamin. In conclusion, our results suggested that sesamin showed anti-inflammatory effects in IL-1β-stimulated chondrocytes by activating Nrf2 signaling pathway.

Citing Articles

The Nrf2/HO-1 pathway participates in the antiapoptotic and anti-inflammatory effects of platelet-rich plasma in the treatment of osteoarthritis.

Du G, Sun X, He S, Mi L Immun Inflamm Dis. 2024; 12(6):e1169.

PMID: 38860757 PMC: 11165680. DOI: 10.1002/iid3.1169.

A randomized double-blind active-controlled clinical trial on the efficacy of topical basil () oil in knee osteoarthritis.

Askari A, Hasheminasab F, Sadeghpour O, Naghizadehd M, Ravansalar S, Iraji A Front Pharmacol. 2024; 15:1377527.

PMID: 38769995 PMC: 11103000. DOI: 10.3389/fphar.2024.1377527.

The promising antioxidant effects of lignans: Nrf2 activation comes into view.

Hassanein E, Althagafy H, Baraka M, Abd-Alhameed E, Ibrahim I, El-Maksoud M Naunyn Schmiedebergs Arch Pharmacol. 2024; 397(9):6439-6458.

PMID: 38695909 PMC: 11422461. DOI: 10.1007/s00210-024-03102-x.

Anti-Osteoarthritis Mechanism of the Nrf2 Signaling Pathway.

Saha S, Rebouh N Biomedicines. 2023; 11(12).

PMID: 38137397 PMC: 10741080. DOI: 10.3390/biomedicines11123176.

Anti-COVID-19, Anti-Inflammatory, and Anti-Osteoarthritis Activities of Sesamin from L.

Huang S, Hsieh C, Ting J, De Castro-Cruz K, Wang C, Lee C Bioengineering (Basel). 2023; 10(11).

PMID: 38002386 PMC: 10669907. DOI: 10.3390/bioengineering10111263.

References

Takada Y, Singh S, Aggarwal B . Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004; 279(15):15096-104. DOI: 10.1074/jbc.M311192200. View

Kobayashi M, Squires G, Mousa A, Tanzer M, Zukor D, Antoniou J . Role of interleukin-1 and tumor necrosis factor alpha in matrix degradation of human osteoarthritic cartilage. Arthritis Rheum. 2005; 52(1):128-35. DOI: 10.1002/art.20776. View

Xu P, Cai F, Liu X, Guo L . Sesamin inhibits lipopolysaccharide-induced proliferation and invasion through the p38-MAPK and NF-κB signaling pathways in prostate cancer cells. Oncol Rep. 2015; 33(6):3117-23. DOI: 10.3892/or.2015.3888. View

Li W, Khor T, Xu C, Shen G, Jeong W, Yu S . Activation of Nrf2-antioxidant signaling attenuates NFkappaB-inflammatory response and elicits apoptosis. Biochem Pharmacol. 2008; 76(11):1485-9. PMC: 2610259. DOI: 10.1016/j.bcp.2008.07.017. View

Ma L, Gong X, Kuang G, Jiang R, Chen R, Wan J . Sesamin ameliorates lipopolysaccharide/d-galactosamine-induced fulminant hepatic failure by suppression of Toll-like receptor 4 signaling in mice. Biochem Biophys Res Commun. 2015; 461(2):230-6. DOI: 10.1016/j.bbrc.2015.03.154. View

Li K, Li Y, Xu B, Mao L, Zhao J . Sesamin inhibits lipopolysaccharide-induced inflammation and extracellular matrix catabolism in rat intervertebral disc. Connect Tissue Res. 2016; 57(5):347-59. DOI: 10.1080/03008207.2016.1182998. View

Schafer M, Werner S . Nrf2--A regulator of keratinocyte redox signaling. Free Radic Biol Med. 2015; 88(Pt B):243-252. DOI: 10.1016/j.freeradbiomed.2015.04.018. View

Abramson S . Nitric oxide in inflammation and pain associated with osteoarthritis. Arthritis Res Ther. 2008; 10 Suppl 2:S2. PMC: 2582805. DOI: 10.1186/ar2463. View

Ahmed S, Wang N, Hafeez B, Cheruvu V, Haqqi T . Punica granatum L. extract inhibits IL-1beta-induced expression of matrix metalloproteinases by inhibiting the activation of MAP kinases and NF-kappaB in human chondrocytes in vitro. J Nutr. 2005; 135(9):2096-102. PMC: 1315308. DOI: 10.1093/jn/135.9.2096. View

10.

Daheshia M, Yao J . The interleukin 1beta pathway in the pathogenesis of osteoarthritis. J Rheumatol. 2008; 35(12):2306-12. DOI: 10.3899/jrheum.080346. View

11.

Dahlberg L, Billinghurst R, Manner P, Nelson F, Webb G, Ionescu M . Selective enhancement of collagenase-mediated cleavage of resident type II collagen in cultured osteoarthritic cartilage and arrest with a synthetic inhibitor that spares collagenase 1 (matrix metalloproteinase 1). Arthritis Rheum. 2000; 43(3):673-82. DOI: 10.1002/1529-0131(200003)43:3<673::AID-ANR25>3.0.CO;2-8. View

12.

Shakibaei M, Csaki C, Nebrich S, Mobasheri A . Resveratrol suppresses interleukin-1beta-induced inflammatory signaling and apoptosis in human articular chondrocytes: potential for use as a novel nutraceutical for the treatment of osteoarthritis. Biochem Pharmacol. 2008; 76(11):1426-39. DOI: 10.1016/j.bcp.2008.05.029. View

13.

Fu Y, Hu X, Cao Y, Zhang Z, Zhang N . Saikosaponin a inhibits lipopolysaccharide-oxidative stress and inflammation in Human umbilical vein endothelial cells via preventing TLR4 translocation into lipid rafts. Free Radic Biol Med. 2015; 89:777-85. DOI: 10.1016/j.freeradbiomed.2015.10.407. View

14.

Baldwin Jr A . The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol. 1996; 14:649-83. DOI: 10.1146/annurev.immunol.14.1.649. View

15.

Qiang L, Yuan J, Shouyin J, Yulin L, Libing J, Jian-An W . Sesamin Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibition of TLR4 Signaling Pathways. Inflammation. 2015; 39(1):467-472. DOI: 10.1007/s10753-015-0270-6. View

16.

Sokolove J, Lepus C . Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Ther Adv Musculoskelet Dis. 2013; 5(2):77-94. PMC: 3638313. DOI: 10.1177/1759720X12467868. View

17.

Pearle A, Warren R, Rodeo S . Basic science of articular cartilage and osteoarthritis. Clin Sports Med. 2005; 24(1):1-12. DOI: 10.1016/j.csm.2004.08.007. View

18.

Yoshihara Y, Nakamura H, Obata K, Yamada H, Hayakawa T, Fujikawa K . Matrix metalloproteinases and tissue inhibitors of metalloproteinases in synovial fluids from patients with rheumatoid arthritis or osteoarthritis. Ann Rheum Dis. 2000; 59(6):455-61. PMC: 1753174. DOI: 10.1136/ard.59.6.455. View

19.

Wang J, Guo C, Wei Z, He X, Kou J, Zhou E . Morin suppresses inflammatory cytokine expression by downregulation of nuclear factor-κB and mitogen-activated protein kinase (MAPK) signaling pathways in lipopolysaccharide-stimulated primary bovine mammary epithelial cells. J Dairy Sci. 2016; 99(4):3016-3022. DOI: 10.3168/jds.2015-10330. View

20.

Wang X, Hunter D, Xu J, Ding C . Metabolic triggered inflammation in osteoarthritis. Osteoarthritis Cartilage. 2014; 23(1):22-30. DOI: 10.1016/j.joca.2014.10.002. View