The SRL Peptide of Rhesus Rotavirus VP4 Protein Governs Cholangiocyte Infection and the Murine Model of Biliary Atresia

Overview

Journal Hepatology

Specialty Gastroenterology

Date 2016 Nov 19

PMID 27859498

Citations 10

Authors

Sujit K Mohanty

Bryan Donnelly

Inna Lobeck

Ashley Walther

Phylicia Dupree

Abigail Coots

Jaroslaw Meller

Monica McNeal

Karol Sestak

Greg Tiao

Affiliations

Soon will be listed here.

Abstract

Conclusion: The tripeptide SRL on RRV VP4 binds to the cholangiocyte membrane protein Hsc70, defining a novel binding site governing VP4 attachment. Investigations are underway to determine the cellular response to this interaction to understand how it contributes to the pathogenesis of BA. (Hepatology 2017;65:1278-1292).

Citing Articles

Rhesus rotavirus NSP1 mediates extra-intestinal infection and is a contributing factor for biliary obstruction.

Li E, Feng N, Zeng Q, Sanchez-Tacuba L, Kawagishi T, Branham G PLoS Pathog. 2024; 20(9):e1012609.

PMID: 39348381 PMC: 11476687. DOI: 10.1371/journal.ppat.1012609.

A G3P[3] bat rotavirus can infect cultured human cholangiocytes and cause biliary atresia symptom in suckling mice.

Hao P, Pang Z, Qu Q, Cui C, Jiang Y, Chen J Virol Sin. 2024; 39(6):974-976.

PMID: 39326847 PMC: 11738784. DOI: 10.1016/j.virs.2024.09.009.

Specific binding sites on Rhesus rotavirus capsid protein dictate the method of endocytosis inducing the murine model of biliary atresia.

Temple H, Donnelly B, Mohanty S, Mowery S, Poling H, Pasula R Am J Physiol Gastrointest Liver Physiol. 2024; 327(2):G267-G283.

PMID: 38860860 PMC: 11687966. DOI: 10.1152/ajpgi.00308.2023.

Identifying and validating molecular subtypes of biliary atresia using multiple high-throughput data integration analysis.

Wang D, Yang S, Zhao Y, Zhang Y, Hua K, Gu Y Front Immunol. 2023; 13:1008246.

PMID: 36713418 PMC: 9878701. DOI: 10.3389/fimmu.2022.1008246.

T-Bet Deficiency Attenuates Bile Duct Injury in Experimental Biliary Atresia.

Mohanty S, Donnelly B, Temple H, Bondoc A, McNeal M, Tiao G Cells. 2021; 10(12).

PMID: 34943969 PMC: 8700492. DOI: 10.3390/cells10123461.

References

Graham K, Halasz P, Tan Y, Hewish M, Takada Y, Mackow E . Integrin-using rotaviruses bind alpha2beta1 integrin alpha2 I domain via VP4 DGE sequence and recognize alphaXbeta2 and alphaVbeta3 by using VP7 during cell entry. J Virol. 2003; 77(18):9969-78. PMC: 224597. DOI: 10.1128/jvi.77.18.9969-9978.2003. View

Frydman J, Hartl F . Principles of chaperone-assisted protein folding: differences between in vitro and in vivo mechanisms. Science. 1996; 272(5267):1497-502. DOI: 10.1126/science.272.5267.1497. View

Jafri M, Donnelly B, Allen S, Bondoc A, McNeal M, Rennert P . Cholangiocyte expression of alpha2beta1-integrin confers susceptibility to rotavirus-induced experimental biliary atresia. Am J Physiol Gastrointest Liver Physiol. 2008; 295(1):G16-G26. PMC: 2494723. DOI: 10.1152/ajpgi.00442.2007. View

Khamrin P, Maneekarn N, Peerakome S, Yagyu F, Okitsu S, Ushijima H . Molecular characterization of a rare G3P[3] human rotavirus reassortant strain reveals evidence for multiple human-animal interspecies transmissions. J Med Virol. 2006; 78(7):986-94. DOI: 10.1002/jmv.20651. View

Arias C, Guerrero C, Mendez E, Zarate S, Isa P, Espinosa R . Early events of rotavirus infection: the search for the receptor(s). Novartis Found Symp. 2001; 238:47-60; discussion 60-3. DOI: 10.1002/0470846534.ch4. View

Eismann T, Huber N, Shin T, Kuboki S, Galloway E, Wyder M . Peroxiredoxin-6 protects against mitochondrial dysfunction and liver injury during ischemia-reperfusion in mice. Am J Physiol Gastrointest Liver Physiol. 2008; 296(2):G266-74. PMC: 2643922. DOI: 10.1152/ajpgi.90583.2008. View

Wang W, Donnelly B, Bondoc A, Mohanty S, McNeal M, Ward R . The rhesus rotavirus gene encoding VP4 is a major determinant in the pathogenesis of biliary atresia in newborn mice. J Virol. 2011; 85(17):9069-77. PMC: 3165802. DOI: 10.1128/JVI.02436-10. View

Lopez S, Arias C . Multistep entry of rotavirus into cells: a Versaillesque dance. Trends Microbiol. 2004; 12(6):271-8. DOI: 10.1016/j.tim.2004.04.003. View

Li K, Lin X, Huang K, Zhang B, Shi M, Guo W . Identification of novel and diverse rotaviruses in rodents and insectivores, and evidence of cross-species transmission into humans. Virology. 2016; 494:168-77. PMC: 7173014. DOI: 10.1016/j.virol.2016.04.017. View

10.

Fjaer R, Bruu A, Nordbo S . Extrahepatic bile duct atresia and viral involvement. Pediatr Transplant. 2005; 9(1):68-73. DOI: 10.1111/j.1399-3046.2005.00257.x. View

11.

Arias C, Isa P, Guerrero C, Mendez E, Zarate S, Lopez T . Molecular biology of rotavirus cell entry. Arch Med Res. 2002; 33(4):356-61. DOI: 10.1016/s0188-4409(02)00374-0. View

12.

LANDING B . Considerations of the pathogenesis of neonatal hepatitis, biliary atresia and choledochal cyst--the concept of infantile obstructive cholangiopathy. Prog Pediatr Surg. 1974; 6:113-39. View

13.

Gualtero D, Guzman F, Acosta O, Guerrero C . Amino acid domains 280-297 of VP6 and 531-554 of VP4 are implicated in heat shock cognate protein hsc70-mediated rotavirus infection. Arch Virol. 2007; 152(12):2183-96. DOI: 10.1007/s00705-007-1055-5. View

14.

Lopez S, Arias C . Early steps in rotavirus cell entry. Curr Top Microbiol Immunol. 2006; 309:39-66. DOI: 10.1007/3-540-30773-7_2. View

15.

Saxena V, Shivakumar P, Sabla G, Mourya R, Chougnet C, Bezerra J . Dendritic cells regulate natural killer cell activation and epithelial injury in experimental biliary atresia. Sci Transl Med. 2011; 3(102):102ra94. PMC: 4006997. DOI: 10.1126/scitranslmed.3002069. View

16.

Hewish M, Takada Y, Coulson B . Integrins alpha2beta1 and alpha4beta1 can mediate SA11 rotavirus attachment and entry into cells. J Virol. 1999; 74(1):228-36. PMC: 111532. DOI: 10.1128/jvi.74.1.228-236.2000. View

17.

Ciechanover A . The ubiquitin-proteasome pathway: on protein death and cell life. EMBO J. 1998; 17(24):7151-60. PMC: 1171061. DOI: 10.1093/emboj/17.24.7151. View

18.

Petersen C, Grasshoff S, Luciano L . Diverse morphology of biliary atresia in an animal model. J Hepatol. 1998; 28(4):603-7. DOI: 10.1016/s0168-8278(98)80283-3. View

19.

Coulson B, Londrigan S, Lee D . Rotavirus contains integrin ligand sequences and a disintegrin-like domain that are implicated in virus entry into cells. Proc Natl Acad Sci U S A. 1997; 94(10):5389-94. PMC: 24688. DOI: 10.1073/pnas.94.10.5389. View

20.

Okuno Y, Imamoto N, Yoneda Y . 70-kDa heat-shock cognate protein colocalizes with karyophilic proteins into the nucleus during their transport in vitro. Exp Cell Res. 1993; 206(1):134-42. DOI: 10.1006/excr.1993.1129. View