Mapping of Functional Subdomains in the Terminal Protein Domain of Hepatitis B Virus Polymerase

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2016 Nov 18

PMID 27852858

Citations 13

Authors

Daniel N Clark

John M Flanagan

Jianming Hu

Affiliations

Soon will be listed here.

Abstract

Importance: HBV is a major cause of viral hepatitis, liver cirrhosis, and hepatocellular carcinoma. One important feature of this virus is its polymerase, the enzyme used to create the DNA genome from a specific viral RNA by reverse transcription. One region of this polymerase, the TP domain, is required for association with the viral RNA and production of the DNA genome. Targeting the TP domain for antiviral development is difficult due to the lack of homology to other proteins and high-resolution structure. This study mapped the TP functions according to predicted secondary structure, where it folds into alpha helices or unstructured loops. Three predicted loops were found to be the most important regions functionally and the most conserved evolutionarily. Identification of these functional subdomains in TP will facilitate its targeting for antiviral development.

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