Molecular Identification of from Seroprevalent Commercial Breeder Farms at Chittagong District, Bangladesh

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2016 Nov 17

PMID 27847414

Citations 1

Authors

Md Inkeyas Uddin

Md Harisul Abid

Md Shafiqul Islam

Tofazzal Md Rakib

Ashim Baran Sen

Shah Mohammed Ziqrul Haq Chowdhury

Md Nurul Anwar

Kazi Md Kamaruddin

Affiliations

Soon will be listed here.

Abstract

Aim: Worldwide, (MS) is an important pathogen of poultry, especially for chicken and turkey. It causes respiratory tract infection and infectious sinusitis. The study was conducted to determine the seroprevalence of MS infection with associated risk factors and identification of MS organism in unvaccinated flocks of commercial breeder farms of the Chittagong district, Bangladesh.

Materials And Methods: A total of 365 serum samples were collected and tested for MS using serum plate agglutination (SPA) test for determination of MS seroprevalence. On the other hand, tracheal swabs were collected from each seropositive flocks for polymerase chain reaction (PCR) to determine the presence of MS organism.

Results: Among the farms, the highest prevalence was found to be 69% and the lowest prevalence was 28% with the average 60%. The seroprevalence of MS infection in breeder farms was highest 70% with the flock size >10,000 birds, whereas it was lowest 57% in the flocks ranging from 4000 to 7000. According to age group, the prevalence was found highest 70% in >60 weeks age group of birds and lowest 42% in 10-19 weeks group. The seroprevalence of MS in winter season was found as highest as 64%, whereas it was found lowest 60% in the summer season. There was a statistically significant difference (p<0.01) among the seroprevalence of MS in different breeder farms, flock size, and age groups, but there was no significant (p>0.05) difference in the winter, summer, and rainy season. To confirm the presence of MS in the samples, PCR test was applied using specific primers to amplify a 214 bp region of the 16S rRNA gene of the organism. In PCR, all seropositive flocks showed a positive result for MS.

Conclusion: As the plate agglutination test result showed 100% similar with PCR result, it can be suggested that agglutination test is better than molecular and culture techniques for MS detection and it is also cheaper and less time-consuming method.

Citing Articles

Molecular identification of from breeder chicken flock showing arthritis in Egypt.

Amer M, Mekky H, Fedawy H Vet World. 2019; 12(4):535-541.

PMID: 31190708 PMC: 6515820. DOI: 10.14202/vetworld.2019.535-541.

References

Hong Y, Garcia M, Leiting V, Bencina D, Dufour-Zavala L, Zavala G . Specific detection and typing of Mycoplasma synoviae strains in poultry with PCR and DNA sequence analysis targeting the hemagglutinin encoding gene vlhA. Avian Dis. 2004; 48(3):606-16. DOI: 10.1637/7156-011504R. View

Landman W . Is Mycoplasma synoviae outrunning Mycoplasma gallisepticum? A viewpoint from the Netherlands. Avian Pathol. 2014; 43(1):2-8. DOI: 10.1080/03079457.2014.881049. View

Hammouda A, Pearce-Duvet J, Boulinier T, Selmi S . Egg sampling as a possible alternative to blood sampling when monitoring the exposure of yellow-legged gulls (Larus michahellis) to avian influenza viruses. Avian Pathol. 2014; 43(6):547-51. DOI: 10.1080/03079457.2014.972340. View

May M, Dunne D, Brown D . A sialoreceptor binding motif in the Mycoplasma synoviae adhesin VlhA. PLoS One. 2014; 9(10):e110360. PMC: 4206340. DOI: 10.1371/journal.pone.0110360. View

Bradbury J, Yavari C, Dupiellet J, Bove J . Mycoplasma imitans sp. nov. is related to Mycoplasma gallisepticum and found in birds. Int J Syst Bacteriol. 1993; 43(4):721-8. DOI: 10.1099/00207713-43-4-721. View

McBride M, Hird D, Carpenter T, Snipes K, Utterback W . Health survey of backyard poultry and other avian species located within one mile of commercial California meat-turkey flocks. Avian Dis. 1991; 35(2):403-7. View

Al-Shekaili T, Baylis M, Ganapathy K . Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry. Res Vet Sci. 2015; 99:46-52. PMC: 7111884. DOI: 10.1016/j.rvsc.2014.12.018. View

Ewing M, Cookson K, Phillips R, Turner K, Kleven S . Experimental infection and transmissibility of Mycoplasma synoviae with delayed serologic response in chickens. Avian Dis. 1998; 42(2):230-8. View

Lauerman L, Hoerr F, Sharpton A, Shah S, van Santen V . Development and application of a polymerase chain reaction assay for Mycoplasma synoviae. Avian Dis. 1993; 37(3):829-34. View

10.

Raviv Z, Kleven S . The development of diagnostic real-time TaqMan PCRs for the four pathogenic avian mycoplasmas. Avian Dis. 2009; 53(1):103-7. DOI: 10.1637/8469-091508-Reg.1. View

11.

Ghorashi S, Kanci A, Noormohammadi A . Evaluation of the Capacity of PCR and High-Resolution Melt Curve Analysis for Identification of Mixed Infection with Mycoplasma gallisepticum Strains. PLoS One. 2015; 10(5):e0126824. PMC: 4430288. DOI: 10.1371/journal.pone.0126824. View

12.

Wilson David S, Volokhov D, Ye Z, Chizhikov V . Evaluation of Mycoplasma inactivation during production of biologics: egg-based viral vaccines as a model. Appl Environ Microbiol. 2010; 76(9):2718-28. PMC: 2863464. DOI: 10.1128/AEM.02776-09. View

13.

Ben Abdelmoumen Mardassi B, Mohamed R, Gueriri I, Boughattas S, Mlik B . Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes. J Clin Microbiol. 2005; 43(2):948-58. PMC: 548031. DOI: 10.1128/JCM.43.2.948-950.2005. View

14.

Bonneaud C, Balenger S, Russell A, Zhang J, Hill G, Edwards S . Rapid evolution of disease resistance is accompanied by functional changes in gene expression in a wild bird. Proc Natl Acad Sci U S A. 2011; 108(19):7866-71. PMC: 3093480. DOI: 10.1073/pnas.1018580108. View

15.

Ogino S, Munakata Y, Ohashi S, Fukui M, Sakamoto H, Sekiya Y . Genotyping of Japanese field isolates of Mycoplasma synoviae and rapid molecular differentiation from the MS-H vaccine strain. Avian Dis. 2011; 55(2):187-94. DOI: 10.1637/9461-071310-Reg.1. View

16.

Catania S, Bilato D, Gobbo F, Granato A, Terregino C, Iob L . Treatment of eggshell abnormalities and reduced egg production caused by Mycoplasma synoviae infection. Avian Dis. 2010; 54(2):961-4. DOI: 10.1637/9121-110309-Case.1. View

17.

Feberwee A, Mekkes D, de Wit J, Hartman E, Pijpers A . Comparison of culture, PCR, and different serologic tests for detection of Mycoplasma gallisepticum and Mycoplasma synoviae infections. Avian Dis. 2005; 49(2):260-8. DOI: 10.1637/7274-090804R. View

18.

Iloh G, Amadi A, Nwankwo B, Ugwu V . Obesity in adult Nigerians: a study of its pattern and common primary co-morbidities in a rural Mission General Hospital in Imo state,South-Eastern Nigeria. Niger J Clin Pract. 2011; 14(2):212-8. DOI: 10.4103/1119-3077.84019. View

19.

Feberwee A, de Vries T, Landman W . Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms. Avian Pathol. 2008; 37(6):629-33. DOI: 10.1080/03079450802484987. View

20.

Stipkovits L, Kempf I . Mycoplasmoses in poultry. Rev Sci Tech. 1996; 15(4):1495-525. DOI: 10.20506/rst.15.4.986. View