Live Monitoring of Blastemal Cell Contributions During Appendage Regeneration

Overview

Journal Curr Biol

Publisher Cell Press

Specialty Biology

Date 2016 Nov 15

PMID 27839971

Citations 30

Authors

Valerie A Tornini

Alberto Puliafito

Leslie A Slota

John D Thompson

Gregory Nachtrab

Anna-Lila Kaushik

Marika Kapsimali

Luca Primo

Stefano Di Talia

Kenneth D Poss

Affiliations

Soon will be listed here.

Abstract

The blastema is a mass of progenitor cells that enables regeneration of amputated salamander limbs or fish fins. Methodology to label and track blastemal cell progeny has been deficient, restricting our understanding of appendage regeneration. Here, we created a system for clonal analysis and quantitative imaging of hundreds of blastemal cells and their respective progeny in living adult zebrafish undergoing fin regeneration. Amputation stimulates resident cells within a limited recruitment zone to reset proximodistal (PD) positional information and assemble the blastema. Within the newly formed blastema, the spatial coordinates of connective tissue progenitors are predictive of their ultimate contributions to regenerated skeletal structures, indicating early development of an approximate PD pre-pattern. Calcineurin regulates size recovery by controlling the average number of progeny divisions without disrupting this pre-pattern. Our longitudinal clonal analyses of regenerating zebrafish fins provide evidence that connective tissue progenitors are rapidly organized into a scalable blueprint of lost structures.

Citing Articles

Decaying and expanding Erk gradients process memory of skeletal size during zebrafish fin regeneration.

Rich A, Lu Z, Simone A, Garcia L, Janssen J, Ando K bioRxiv. 2025; .

PMID: 39896678 PMC: 11785216. DOI: 10.1101/2025.01.23.634576.

Negative cell cycle regulation by calcineurin is necessary for proper beta cell regeneration in zebrafish.

Massoz L, Bergemann D, Lavergne A, Reynders C, Desiront C, Goossens C Elife. 2024; 12.

PMID: 39383064 PMC: 11464004. DOI: 10.7554/eLife.88813.

Growth patterns of caudal fin rays are informed by both external signals from the regenerating organ and remembered identity autonomous to the local tissue.

Autumn M, Hu Y, Zeng J, McMenamin S Dev Biol. 2024; 515:121-128.

PMID: 39029570 PMC: 11361315. DOI: 10.1016/j.ydbio.2024.07.008.

Growth patterns of caudal fin rays are informed by both external signals from the regenerating organ and remembered identity autonomous to the local tissue.

Autumn M, Hu Y, Zeng J, McMenamin S bioRxiv. 2024; .

PMID: 38585773 PMC: 10996721. DOI: 10.1101/2024.03.29.586899.

A screen for regeneration-associated silencer regulatory elements in zebrafish.

Ando K, Ou J, Thompson J, Welsby J, Bangru S, Shen J Dev Cell. 2024; 59(5):676-691.e5.

PMID: 38290519 PMC: 10939760. DOI: 10.1016/j.devcel.2024.01.004.

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