In Vitro Fixation of C3d and C5b-9 on Platelets by Human Platelet Reactive Antibodies

Overview

Journal Blut

Specialty Hematology

Date 1989 Jan 1

PMID 2783867

Citations 3

Authors

V Kiefel

A Salama

C MUELLER-ECKHARDT

Affiliations

Soon will be listed here.

Abstract

Most platelet-reactive autoantibodies and alloantibodies are not able to fix complement in vitro. However, exceptions have been found. These antibodies are usually characterized by the conventional platelet complement fixation test. A recently developed competitive enzyme immunoassay for quantitation of platelet-associated immunoglobulins and a modification thereof allowed the quantitative study of fixation of C3d and the membrane attack complex (C5b-9) on platelets by HLA antibodies, human platelet autoantibodies, and drug-dependent antibodies (ddab). The highest amounts of both complement products were fixed through ddabs, whereas autoantibodies only showed moderate complement fixation. This enzyme immunoassay is a valuable tool for the characterization of the complement-fixing properties of platelet-reactive antibodies.

Citing Articles

Pathophysiology and Diagnosis of Drug-Induced Immune Thrombocytopenia.

Vayne C, Guery E, Rollin J, Baglo T, Petermann R, Gruel Y J Clin Med. 2020; 9(7).

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Recent trends in platelet antigen/antibody detection.

MUELLER-ECKHARDT C, Kiefel V, Santoso S Blut. 1989; 59(1):35-43.

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Antiplatelet autoantibody-related microparticles in patients with idiopathic (autoimmune) thrombocytopenic purpura.

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